PcDNA3.1/sTNFRⅠ重组质粒在CHO细胞内的表达  

Expression of the Recombinant Plasmid PcDNA3.1/sTNFRⅠ in CHO Cells

作　　者：徐琛蓉[1] 章锦才[1] 赵川江[2] 张蕴惠[3] 庞若愚[1] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院牙周科,广东广州510280 [2]中山大学附属光华口腔医学院牙周黏膜科 [3]四川大学华西口腔医院牙周科

出　　处：《广东牙病防治》2006年第1期13-15,共3页Journal of Dental Prevention and Treatment

基　　金：广东省医学科学技术研究基金资助项目(A2004119)

摘　　要：目的检测构建的PcDNA3.1/sTNFRⅠ真核表达载体在体外转染中国仓鼠卵巢(CHO)细胞后能否有效表达目的产物。方法ELISA法检测PcDNA3.1/sTNFRⅠ转染的CHO细胞培养上清液中sTNFRⅠ的表达。结果重组质粒转染的CHO细胞上清液中表达的sTNFRⅠ量超过1 000 pg/mL,显著高于对照组(109.6±5.32)pg/mL。结论PcDNA3.1/sTNFRⅠ重组质粒在体外导入哺乳动物细胞后能够表达相应的目的产物。Objective To detect the expression of recombinant plasmid PcDNA3.1/sTNFR Ⅰ in vitro. Methods CHO ceils were transfected with recombinant plasmid PcDNA3.1/sTNFRⅠ by lipesome, sTNFR Ⅰ expressed in cell culture supematant was detected by ELISA. Results It was much higher of sTNFR Ⅰ in transfected cell culture supernatant than control groups. Conclusion These results showed that the recombinant plasmid PcDNA3.1/sTNFR Ⅰ can be expressed in mammalian cells.

关 键 词：Ⅰ型人可溶性肿瘤坏死因子受体 质粒 转染 中国 仓鼠 卵巢细胞 

分 类 号：R512.62[医药卫生—内科学] R734.2[医药卫生—临床医学]