治疗超声介导微泡造影剂对体外血栓的助溶研究  

The Efficacy of Microbubbles Augmentation Thrombolysis using Therapeutic Ultrasound:an in Vitro Study

作　　者：徐亚丽[1] 刘政[1] 高云华[1] 谭开彬[1] 

机构地区：[1]解放军第三军医大学新桥医院超声科,重庆市400037

出　　处：《中国超声医学杂志》2006年第2期81-83,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然基金资助项目(No.30200265)

摘　　要：目的 运用本科自制脂膜氟烷超声造影剂“脂氟显”结合治疗超声对组织纤溶酶原激活物（tissue-plasminogen activator，t-PA）助溶体外血栓的有效性进行研究。方法 取健康人全血制成质量约200～300mg血栓，频率1MHz治疗超声结合“脂氟显”和t-PA进行溶栓治疗。计算治疗前后的血栓质量，得到溶栓率，进行统计学分析。结果 组间比较溶栓率超声＋t-PA＋“脂氟显”组（50．05±6．59）％与单纯超声辐照（24．14±3．93）％、单纯t-PA组（35．66±3．34）％、超声＋t-PA组（41．85±4．78）％及超声＋“脂氟显”组（29．51％±5．17）％间差异均有十分显著性意义（P〈0．01）；超声＋“脂氟显”组与单纯超声辐照组间比较差异有显著性意义（P〈0．05）。结论 微泡超声造影剂在治疗超声介导下对t-PA助溶体外血栓有显著效果。Objective To promote t-PA thrombolysis in vitro by using lipid-coated micro bubbles （MB） augmented therapeutic ultrasound in sonication. Methods Tbrombolytic efficacy of continuous-wave therapeutic ultrasound （US, 1 MHz） was evaluated. Fresh human blood clot （aged 5-6 h, weight 200～300 mg） were sonicated for 10 min at an intensity of 1.0 W/cm^2. The thrombolytic effect of US alone and in combination with t-PA or MB was assessed with or without addition of MB or t-PA. Loss in weight of each blood clot was used to measure the extent of thrombolysis. Results Compared with taking US （24.14%± 3.93%） or t-PA （35.66%±3.34%） alone, the clot dissolution ratios rised to 41.85%±4. 78% after exposure to US and t-PA （P〈0.01, P〈0. 05）, 50.05%±6.59% after exposure to US, t-PA and MB （P〈0.01, P〈0. 01）. Compared with US alone, the clot dissolution rate rised to 29.51%±5.17% using US and MB （P〈0. 05） .Conclusions The application of lipid-coated MB strongly accelerates iysis of clots exposed to 1.0 MHz, 1.0 W/cm^2 US in vitro both with or without t-PA.

关 键 词：超声 微泡造影剂 溶栓 组织纤溶酶原激活物 

分 类 号：R454[医药卫生—治疗学]