胎儿毛乳头细胞分化为类成骨细胞的实验研究  

Experimental study on fetal dermal papilla cells differentiate into osteogenic cells

作　　者：祁少海[1] 刘坡[1] 谢举临[1] 徐盈斌[1] 盛蹼义 舒斌[1] 利天增[1] 

机构地区：[1]中山大学附属第一医院烧伤科,广州市510080 [2]中山大学附属第一医院骨科

出　　处：《中华显微外科杂志》2006年第1期39-41,i0003,共4页Chinese Journal of Microsurgery

基　　金：广东省自然科学基金资助(31685);广东省科技计划项目基金资助(2004B35001002)

摘　　要：目的研究胎儿毛乳头细胞的体外成骨能力,为骨组织工程提供一种新的种子细胞。方法Ⅰ型胶原酶消化法分离毛乳头,常规成纤维细胞培养液培养毛乳头细胞。第3代和第7代细胞经成骨诱导液诱导培养7d,以免疫荧光、VonKossa等方法鉴定毛乳头细胞及检测细胞的成骨特性。结果培养的毛乳头细胞呈聚集性生长,表达平滑肌肌动蛋白和Ⅰ型胶原。经成骨诱导后表达骨桥蛋白,有钙结节形成。结论毛乳头细胞具有成骨活性,可作为骨组织工程的种子细胞。Objective To explore the osteogenic potential of cultured fetal dermal papilla cells in vitro and provide a new ideal seeded cells for bone tissue engineering. Methods Dermal papilla were isolated by the digestion method of collagenase I and cultured in routine fibroblast medium. Identified the osteogeneric capability of the third and seventh passage cells by immunofluorescence and Von Kossa after 7 clays of culture in osteogenic medium, Results The cuhiveated dermal papilla cells showed distinct tendency to form clumps at confluence, and expressed high levels of a-smooth muscle actin and type I collagen. Cultured in osteogenic medium, those cells produce calcified deposits and express osteopontin. Conclusion The cultivated dermal papilla cells has osteogeneric capability, and could become a new seeded cells for bone tissue engmeermg.

关 键 词：毛乳头细胞 成骨性细胞 骨 组织工程 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]