移植前低钾右旋糖苷液二次灌洗减轻供肺再灌注损伤  

Administering a second rinse solution of low-potassium-dextran before reperfusion ameliorates the donor lung

作　　者：王平善[1] 吴清玉[1] 关彬[1] 史世勇[1] 

机构地区：[1]中国协和医科大学阜外心血管病医院器官移植研究室体外循环研究室

出　　处：《中国医师杂志》2006年第1期27-29,共3页Journal of Chinese Physician

摘　　要：目的观察移植前低钾右旋糖苷液(LPD)二次灌洗对供肺再灌注损伤的保护作用。方法24只大鼠随机分为对照组(n=12)和实验组(n=12),对照组供肺经LPD液灌洗后于4℃保存18 h,取右肺组织测丙二醛(MDA)、髓过氧化物酶(MPO)、白细胞介素-8(IL-8),建立离体肺再灌注模型,左肺灌注30 m in;实验组供肺灌注前用LPD液再灌洗一次,余同对照组。灌注期间每隔10m in测定供肺静脉流出血氧分压(PaO2)、供肺平均肺动脉压(PaP)、气道峰压(PawP),灌注结束后测肺组织丙二醛(MDA)、髓过氧化物酶(MPO)、白细胞介素-8(IL-8)的含量。结果实验组供肺右肺组织MDA、MPO、IL-8含量明显低于对照组,差异有统计学意义(P<0.01);再灌注30m in后实验组供肺PO2明显高于对照组,PaP、PawP明显低于对照组,差异有统计学意义(P<0.01);灌注后供肺组织MDA、MPO、IL-8含量,实验组明显低于对照组,差异有统计学意义(P<0.01)。结论移植前LPD液二次灌洗可以减少供肺组织内MDA、MPO、IL-8含量,减轻供肺的再灌注损伤,改善供肺的功能。Objective To studied the effects of administering a second rinse solution of low-potassium-dextran （LPD） before reperfusion on the donor lung of rat after hmg time, cold ischemie preservation. Methods Twenty-four rats were randomly divided into control group （ n = 12） and trial group （ n = 12）. Donor lungs of control group were flushed with LPD solution. The levels of mahmaldehyde （ MDA）, myeloperoxidase （MPO） and interleukin-8 （ IL-8 ） were tested in the tissue of right lung and the left lungs were reperfused with venous blood for 30 minutes. The partial pressure of nxygen （ PaO2 ）, PaP anti PawP were measured at every 10 minutes intervals during reperfusion. After reperfusion, the MDA, MPO activity and interlakin-8 （IL-8） were determined in the lung tissue. Results The levels of MDA, MPO and IL-8 decreased significantly in right lung of trial group than those in control group （ P 〈 0. 01 ）. After reperfusion of 20 minutes, the PaO2, were higher in trial group than that in control group, and PaP, PawP were lower in trial group than those of control group （ all P 〈0. 01 ）. After reperfusion, the MDA, MPO anti IL-8 decreased significantly in left lung of trial group than in control group （ all P 〈 0. 01 ）. Conclusion A second flush with LPD solution before reperfusion can decrease the levels of MDA, MPO and IL-8, and thus improves the function of donor lungs.

关 键 词：肺移植 再灌注损伤 右旋糖酐类 灌洗 

分 类 号：R655.3[医药卫生—外科学]