联合检测抗核小体抗体和抗dsDNA抗体对SLE疾病活动和脏器损害的意义  

Significance of combination of serum anti-nucleosome and anti-dsDNA antibodies in patients with systemic lupus erythematosus

作　　者：汪国生[1] 张宏[1] 单曙光[1] 杨国俊[1] 钱龙[1] 王玮[1] 厉小梅[1] 李向培[1] 

机构地区：[1]安徽医科大学附属省立医院安徽省立医院风湿科,安徽合肥230001

出　　处：《中国医师杂志》2006年第1期54-56,共3页Journal of Chinese Physician

基　　金：安徽省临床医学重点学科技术进步专项课题(2004Z003)

摘　　要：目的探讨联合检测抗核小体抗体(AnuA)、抗dsDNA抗体对系统性红斑狼疮(SLE)疾病活动和脏器损害的意义。方法69例SLE分为疾病活动和疾病稳定组、狼疮肾炎(LN)和非狼疮肾炎组(NLN)。测定AnuA和抗dsDNA抗体水平,比较组间抗体水平差异,分析其与免疫指标的相关性。结果AnuA和抗dsDNA抗体阳性率为55.1%和47.82%,疾病活动组显著高于疾病稳定组(P<0.001)。抗dsDNA抗体阴性SLE中有16.6%AnuA阳性。两抗体与SLE疾病活动指数(SLEDAI)、IgG、球蛋白水平显著正相关,与C3、C4、白蛋白水平显著负相关。LN组两抗体水平均显著高于NLN。结论AnuA和抗dsDNA抗体都是SLE疾病活动指标,与LN相关,AnuA检测是对抗dsDNA抗体的有益补充。Objective To explore the significance of anti-nucleosome and anti-dsDNA antibodies on the disease flare and organ damage in patients with systemic lupus erythematosus （SLE）. Methods Sixty-nine SLE patients were divided into disease flare group, stable group, lupus nephritis group and non-lupus nephritis group. Serum levels of anti-nucleosome antibody and anti-dsDNA antibody were measured with enzyme linked immunosorbent assay （ELISA） and indirect immunofluorescence （ IIF）, respectively. Results The positive rates of serum anti-nucleosome and anti-dsDNA antibodies were 55. 10% and 47.82% respectively and the rates were significantly higher in disease flare group than those in disease stable group （ P 〈 0. 001 ）. The positive rate of anti-nucleosome antibody may be positive in 16. 6% anti-dsDNA negative patients. The levels of anti-nucleosome and anti-dsDNA antibodies were positively correlated with SLEDAI, IgG and globulin and negatively with the levels of C3, C4 and albumin. Serum levels of anti-nuclesome and anti-dsDNA antibodies were higher in lupus nephritis group than those in non-lupus nephritis group. Conclusion The levels of anti-nucleosome and anti-dsDNA antibodies are index of disease activity and correlated with lupus nephritis. The detection of anti-nucleosome antibody is helpful for the diagnose of SLE patients with negative anti-dsDNA.

关 键 词：红斑狼疮 系统性 抗体 抗核 核小体 DNA 

分 类 号：R593.241[医药卫生—内科学] R734.2[医药卫生—临床医学]