大鼠急性心肌损伤与CT-1mRNA相关性的实验观察  

Experimental study on of the correlation between rat myocardial injury and CT-1 mRNA

作　　者：顾明[1] 白冰[2] 张旭[3] 周令望[1] 刘阳[1] 邹宁[1] 王铜[1] 于维汉[1] 

机构地区：[1]哈尔滨医科大学克山病研究所,150081 [2]哈尔滨医科大学第一临床医学院 [3]黑龙江省医院门诊物理诊断科

出　　处：《中国地方病学杂志》2006年第1期22-24,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目(30271153);黑龙江省自然科学基金资助项目(D2004-22);黑龙江省教育厅资助项目(10531101)

摘　　要：目的研究心肌营养素1(cardiotrophin-1,CT-1)在异丙基肾上腺素(isoprenaline,Iso)致大鼠急性心肌损伤中的保护作用。方法将Wistar雄性大白鼠随机分为3组:对照组、Iso组和Iso+PD98059组,光镜观察心肌组织的病理变化,生化方法检测心肌酶,用RT-PCR方法检测心肌组织CT-1mRNA的表达。结果光镜下Iso+PD98059组心肌病理损伤程度较Iso组重,且CT-1mRNA表达水平及心肌酶活性明显升高(P<0.05)。结论CT-1具有心肌保护作用,可能与减轻心肌细胞坏死的程度有关。Objective To study the protective effects of cardiotrophin-1 （CT-1） on rat myocardial injury induced by isoprenaline （Iso）. Methods Wistar rats were divided into three groups at random, being control group, Iso group and Iso ＋ PD98059 group respectively. The pathological changes of myocardium were observed under microscope. Levels of myocardial enzyme were detected by biochemical method. The expressions of CT-1 mRNA were tested with RT-PCR method. Results In the Iso ＋ PD98059 group, myocardial necrosis was more serious than that in the Iso group,. The expression of CT-1 mRNA and the levels of myocardial enzyme were increased obviously （P 〈 0.01 or P 〈 0.05）as well. Conclusion CT-1 plays a protective role for myocardium, possibly through alleviating the level of myocardial cell necrosis.

关 键 词：心肌营养素1 异丙基肾上腺素 心肌 大鼠 

分 类 号：R541[医药卫生—心血管疾病]