Midkine对PFSK细胞的定向分化诱导作用  

DIRECTIONAL DIFFERENTIATION OF PFSK CELLS INDUCED BY MIDKINE

作　　者：唐以太[1] 徐平[1] 陈献华[1] 刘磊[1] 林万敏[1] 

机构地区：[1]复旦大学生命科学学院生理学及生物物理学系,上海200433

出　　处：《神经解剖学杂志》2006年第1期45-49,共5页Chinese Journal of Neuroanatomy

摘　　要：本研究的目的是观察midkine基因对PFSK细胞的定向诱导作用。通过分子克隆和转染,将人midkine基因导入具有神经前体细胞可分化特性的PFSK细胞,用显微镜观察细胞的形态变化,并以RT-PCR对神经细胞相关基因的表达进行检测,研究midkine诱导的PFSK细胞分化。结果表明,PFSK细胞被转化后,细胞的突起生长明显。同时细胞内MAP-2和NCAM基因的表达趋向活跃;nestin基因的表达大大减弱;GFAP基因仍检测不到。所有这些结果一致地表明,转染midkine后,PFSK细胞呈现向神经元、而不是向神经胶质细胞转化的趋势。本研究对阐明midkine的功能及建立PFSK/midkine研究模式具有一定的意义。The aim of this study was to investigate the directional differentiation of PFSK cells induced bv midkine. Through molecular cloning and gene transfection, the human midkine gene was introdueed into PFSK cells which may differentiate like neural progenitor cells. And the differentiation of PFSK cells hy midkine was studied by observing the ehanges in their morphology under microscope and in the expressions of neuro-related genes with RT-PCR. The results showed that transformed PFSK eells grew with obvious neurites. In addition, compared with control cells, transformed cells showed more active expressions of MAP-2 gene and NCAM gene, much lower nestin gene expression, and the same undeteetable GFAP gene expression. All the changes coincidently suggested the differentiation trend of the PFSK cells after midkine transfection to neurons, not to gliacytes. This result is meaningful for both the clarifying the function of midkine and the estahlishment of PFSK/midkine model.

关 键 词：MIDKINE PFSK细胞 RT-PCR 神经分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]