补阳还五汤提取液对Schwann细胞氧化损伤的保护作用  

THE PROTECTIVE EFFECTS OF BUYANGHUANWU DECOCTION ON THE OXIDATIVE INJURIED SCHWANN CELL

作　　者：邢三丽[1] 李振华[1] 孙晋浩[1] 刘岳鹏[1] 暴丽华[1] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012

出　　处：《神经解剖学杂志》2006年第1期95-98,共4页Chinese Journal of Neuroanatomy

基　　金：山东省自然科学基金(Y2000C14);山东省优秀中青年科学家科研奖励基金(2004BS02012)资助项目

摘　　要：本实验建立了Schwann细胞的体外氧化损伤模型,从对Schwann细胞的抗氧化损伤作用探讨补阳还五汤的疗效机制。用原代培养的Schwann细胞建立氧化损伤模型,将培养细胞分为氧化损伤组,补阳还五汤处理组及正常组:(1)应用MTT方法检测细胞的活性;(2)应用生化技术检测细胞内超氧化物歧化酶(SOD)的含量;(3)采用荧光探针Fluo-3-AM标记细胞,用激光共聚焦显微镜观察H_2O_2对Schwann细胞内游离钙([Ca2+])抗氧化损伤的影响。结果显示:与对照组相比,H2O2处理组细胞活性降低(P<0.01),SOD含量明显减少(P<0.01),而补阳还五汤预处理后细胞内[Ca2+]i升高明显被减弱(P<0.01),细胞存活率明显升高(P<0.01)。以上结果表明补阳还五汤能通过抗过氧化损伤保护Schwann细胞。To investigate the protective effects of Buyanghuanwu Decoction on the oxidative injuried Schwann cell;after making the oxidative injuried model of the cultured Schwann cell ： （ 1 ） MTT was used to observe cell activity of the Schwann cell treated with hydrogen peroxide; （2） Detecting the intracellular superoxide dismutase （SOD） ; （3） The fluorescent probe Fluo-3-AM was used to detect the intracellular Ca^2＋ levels by using Lasers Scanning Confocal Microscopy （LSCM）. The results showed that, compared with the control group, the oxidative injured Schwann cells had decreased cell activity and elevated intracellular Ca^2＋ level. However, these changes were significantly inversed by Buyanghuanwu Decoction preconditioned group. These results suggest that Buyanghuanwu Decoction has good antioxidated effect to protect the Schwann cells.

关 键 词：SCHWANN细胞 氧化损伤 抗氧化作用 补阳还五汤 

分 类 号：R285[医药卫生—中药学]