大黄酸抑制肾间质成纤维细胞激活的实验研究  

Rhein arrests TGF-β1-induced renal interstitial fibroblast activation

作　　者：何东元[1] 王笑云[1] 王宁宁[1] 李瑾[1] 王晓华[1] 郑东辉[1] 任胜利[1] 杨俊伟[1] 

机构地区：[1]南京医科大学第一附属医院肾脏科,210029

出　　处：《中华肾脏病杂志》2006年第2期105-108,共4页Chinese Journal of Nephrology

摘　　要：目的研究大黄酸(Rhein)抑制转化生长因子β1(TGF-β1)激活大鼠肾间质成纤维细胞(NRK-49F)作用及探讨大黄治疗肾脏病的作用机制。方法以NRK-49F细胞为研究对象,采用细胞计数观察细胞生长,流式细胞仪分析细胞周期变化。α-平滑肌肌动蛋白(α-SMA) 作为细胞活化指标,同时检测纤连蛋白(FN)表达的水平以评价细胞外基质合成的变化。结果 TGF-β1具有促进NRK-49F增殖的作用,大黄酸呈时间依赖性和剂量依赖性抑制该增殖作用。 TGF-β1能够促进NRK-49F进入S期和G2／M期,而经大黄酸作用的细胞主要是G0／G1期细胞。大黄酸呈剂量依赖性显著抑制TGF-β1诱导的NRK-49F细胞α-SMA蛋白表达水平,同时, 大黄酸还可减少TGF-β1诱导的NRK-49F细胞FN蛋白的表达水平。结论大黄酸能够阻止 TGF-β1诱导的肾间质成纤维细胞由G0／G1期进入S期和G2／M期,抑制该细胞的增殖。同时, 大黄酸还具有抑制TGF-β1激活肾间质成纤维细胞的作用,并拮抗TGF-β1导致的FN表达与合成。Objective To explore the effect of Rhein on TGF-β1-induced renal interstitial fibroblast activation. Methods Normal rat renal interstitial fibroblast （NRK-49F） treated by TG-β1 were cultured with or without Rhein at different concentrations. Cell number was counted, and cell cycle was analyzed by flow cytometry. The fibroblast activation was assessed by expression of a-smooth muscle actin （α-SMA）, and deposition of fibronectin （FN） by Western blotting. Results Rhein suppressed TGF-β-induced fibroblast growth in time-and dose-dependent manner, and blocked resting cells into S and G2/M phase. It was demonstrated that Rhein abolished α-SMA expression of fibroblast induced by TGF-β1 in a dose-dependent manner. Meanwhile, Rhein suppressed TGF-β1- induced FN deposition and its extracellular assembly in fibroblast. Conclusion Rhein can block renal interstitial fibroblast growth and activation, and the effect of Rhubarb on delaying progression of CRF may be associated with this action of Rhein.

关 键 词：大黄酸 成纤维细胞 转化生长因子Β1 

分 类 号：R285.5[医药卫生—中药学]