海藻糖载入血小板的研究  

Study on Loading Platelets with Trehalose

作　　者：周新丽[1] 祝宏[2] 张绍志[1] 朱发明[2] 陈光明[1] 严力行[2] 

机构地区：[1]浙江大学制冷与低温研究所低温生物实验室,杭州310027 [2]浙江省血液中心,杭州310006

出　　处：《细胞生物学杂志》2006年第1期107-111,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.50076039);卫生部科学研究基金(No.WKJ2005-2-037)资助项目~~

摘　　要：将不可渗透型的保护剂海藻糖有效地载入血小板内部是用冷冻干燥法保存血小板重要的第一步。研究血小板对海藻糖的载入量随外部海藻糖浓度、孵化时间、孵化温度改变的变化规律,发现在细胞外海藻糖浓度为50mmol/L、孵化温度37℃、孵化时间4h的条件下,血小板能有效地吸收海藻糖,细胞内海藻糖浓度达到15mmol/L以上。对孵化后的血小板进行形态观察、血液学分析和膜联蛋白(annexin)V结合活化分析,结果表明孵化后的血小板保持了正常血小板的形态和功能。Introducing the impermeable lyoprotectant trehalose into the cytoplasm is the first step toward preserving platelets by freeze-drying. We investigated the loading of the human platelets with trehalose. Trehalose uptake was measured as a function of extracellular trehalose concentration, incubation time and incubation temperature. We found an effective uptake of the trehalose at 37 ℃ in a time span of 4 h in the presence of 50 mmol/L external trehalose. The internal trehalose concentration reached above 15 mmol/L. Morphological study, hematology analysis and annexin V binding assay showed that trehalose loaded platelets maintain the normal morphology and function of platelets. These data provide the foundation in developing a protocol for freeze-drying platelets.

关 键 词：血小板 海藻糖 载入 冷冻干燥 

分 类 号：Q813[生物学—生物工程]