胰岛素对糖尿病大鼠海马内神经营养因子-3阳性神经元表达的影响  

Effect of insulin on changes of neurotrophin-3 positive neurons in hippocampus of diabetic rats

作　　者：侯立胜[1] 韩卉[1] 贾雪梅[2] 王盛花[2] 吴学平[2] 张孝林[3] 汪渊[3] 

机构地区：[1]安徽医科大学解剖学教研室,合肥230032 [2]安徽医科大学形态学中心实验室,合肥230032 [3]安徽医科大学 分子生物学中心实验室,合肥230032

出　　处：《解剖学杂志》2006年第1期81-84,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(39870324)安徽省学术与技术带头人科研基金

摘　　要：目的：观察糖尿病大鼠海马中神经营养因子-3(NT-3)的表达变化及胰岛素治疗对其表达的影响。方法：将雄性大鼠随机分为对照组,糖尿病1,3月组,胰岛素治疗1,3月组,STZ 腹腔注射制模,测体重与血糖值并取海马行 HE 和免疫组化 SABC 法染色,计算机图像分析系统测平均光密度值。结果：血糖在糖尿病组比对照组显著升高；胰岛素组与对照组无显著性差异。海马各区 NT-3免疫阳性神经元的数量及阳性强度在糖尿病组有不同程度降低,以 CA1区最明显。胰岛素组则不降低。结论：糖尿病大鼠海马神经元 NT-3表达减弱,胰岛素治疗可使海马神经元 NT-3表达恢复正常。Objective： To observe the changes of neurotrophin-3 （NT-3） expression in diabetic rat hippocarnpal neurons and the effect of insulin on its expression. Methods： Thirty male SD rats were divided into normal control group, 1 month diabetic group, 3 months diabetic group, 1 month insulin group and 3 months insulin group. Diabetic rats was induced by intraperitoneal injection of STZ Body weight and blood glucose of rats were detected at the end of 1 month and 3 months. The hippocarnpus was isolated for HE and SABC staining to detect NT-3 positive neurons. Results： Compared with normal groups, the weight and blood glucose of experimental groups changed obviously. The weight and blood glucose of insulin group had no obvious change with that of normal group. The quantity and intensity of positive neurons declined in each subunit of hippocarnpus, especially in CA3 subunit. NT-3 positive neurons in insulin groups did not decline. Conclusion： NT-3 expression is down-regulated in hippocampus of diabetic rat and this decrease can be inhibited by insulin.

关 键 词：糖尿病 神经营养因子-3 海马 大鼠 胰岛素 

分 类 号：R587.1[医药卫生—内分泌]