一粒系小麦的微卫星分析  

SSR Analysis of Einkorn Wheats

作　　者：白建荣[1] 宋彦霞 朱银峰[2] 刘坤凡[2] 贾旭[2] 

机构地区：[1]山西省农业科学院作物遗传研究所,山西太原030031 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《华北农学报》2005年第4期12-16,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展规划项目(G19990116-02)

摘　　要：利用普通小麦A基因组含有微卫星位点的引物对在3种一粒系小麦的部分同源微卫星位点进行了检测。结果发现,59%的小麦A基因组微卫星位点的引物对可在3种一粒系小麦的多个种质中检测到部分同源微卫星位点,表明A基因组含有的微卫星位点在普通小麦和一粒系小麦间并不完全保守。一粒系小麦的微卫星位点之间在扩增条带数目以及扩增条带长度上均存在广泛差异,同一微卫星位点在不同一粒系小麦种质中的扩增条带数目以及扩增条带长度上也表现明显差异。The presence in einkorn wheats of mierosatellite loci homoeologous to those in the A genome of hexaploid wheat were studied. Up to 59 % of the PCR primer sets for the microsatellite loci of the A genome of hexaploid wheat detected homoeologous loci in multiple accessions of T. urartu, T. monococcum and T. boeoticum, indicating that A genome encoded microsatellite loci are highly, but not completely, conserved between hexaploid wheat and einkorn wheats. Microsatellite loci in einkorn wheats differed from each other in both allele number and the length of individual alleles. For a given locus, its allele number and allele length could also vary among different accessi3ns of T. urartu, T. monococcum or T. boeoticum.

关 键 词：一粒系小麦 微卫星 

分 类 号：S512.1[农业科学—作物学]