一种大肠杆菌胆碱脱氢酶活性的测定方法  

A method for measuring the enzyme activity of E.coli Choline dehydrogenase

作　　者：李佳忠[1] 王春明[2] 袁金铎[1] 安利国[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]聊城大学生物系,山东聊城252000

出　　处：《山东科学》2006年第1期32-34,49,共4页Shandong Science

摘　　要：本文提出了一种简单易行的测定微生物中胆碱脱氢酶活力的方法。通过测定549nm波长下电子传递体系PMS(吩嗪二甲酯硫酸盐)-cty.c(细胞色素C)光吸收值的增加,就可以计算出胆碱脱氢酶活力。利用此方法测定了大肠杆菌胆碱脱氢酶热激诱导前后的活力,结果发现受到诱导的大肠杆菌中胆碱脱氢酶的活力明显高于对照组。This paper investigated a simple and available method for measuring the enzyme activity of Choline dehydrogenase（CDH） in microorganisms, it can be used as a parameter to estimate the synthesis of betaine in organisms. The enzyme activity of CDH can be calculated by measuring the absorption of cty. c at 549nm in electron transportation system PMS-cty. c. The result showed that the enzyme activity of the induced E. coli was higher than those of the control group＇ s obviously.

关 键 词：大肠杆菌 胆碱脱氢酶 酶的活力 酶的比活力 

分 类 号：Q554.9[生物学—生物化学]