日本血吸虫感染新模型小鼠Th1/Th2细胞因子水平的动态变化  

DYNAMICS CHANGES OF Th1/Th2 CYTOKINE LEVELS IN THE NEW MOUSE MODELS INFECTED WITH SCHISTOSOMA JAPONICUM

作　　者：陈辉[1] 何立[1] 蒋明森[1] 杨孟祥[1] 张培喜[1] 

机构地区：[1]武汉大学医学院病原生物学系寄生虫学教研室,湖北武汉430071

出　　处：《中国寄生虫病防治杂志》2005年第6期412-415,共4页Chinese Journal of Parasitic Disease Control

基　　金：国家自然科学基金资助项目(No.30471509);湖北省科技攻关项目(No.2003AA3003B04);湖北省卫生厅科研基金重点项目(No.JX1A11)

摘　　要：目的在建立日本血吸虫感染小鼠伴随免疫新模型的基础上,通过观察比较新模型小鼠与常规感染小鼠脾细胞经不同抗原刺激后诱生的Th1细胞因子(IFN-γ)和Th2细胞因子(IL-4)水平的动态变化,探讨日本血吸虫感染小鼠伴随免疫新模型的细胞免疫机制. 方法小鼠感染日本血吸虫尾蚴20 d后,按300 mg/kg·d腹腔注射酚酶抑制剂丙烯基硫脲,持续抑制雌虫产卵,建立日本血吸虫感染小鼠伴随免疫新模型.同时设立常规模型对照组和正常小鼠对照组.在感染后第0、3、6、9和12周,取各组小鼠脾细胞进行体外培养,并分别用可溶性成虫抗原(SWAP)、可溶性虫卵抗原(SEA)及非特异性抗原刀豆素(ConA)诱导脾细胞产生细胞因子,应用ELISA方法测定培养上清液中IFN-γ和IL-4的含量. 结果新模型组与常规模型组IFN-γ和IL-4变化趋势基本一致,其中IFN-γ均在感染后4～6周开始上升,7～9周达到高峰,10～12周逐渐下降并恢复至感染前水平;IL-4均在感染后第6周开始逐渐升高,直至12周仍维持高水平;而正常组IFN-γ和IL-4均无明显变化.新模型组经SEA刺激后产生的IFN-γ水平,以及经SWAP和SEA刺激后产生的IL-4水平均显著低于常规模型组(P<0.05);而经SWAP刺激产生的IFN-γ水平显著高于常规模型组(P<0.05).在3种不同的抗原中,ConA组产生的IFN-γ和IL-4水平均显著高于SEA组和SWAP组(P<0.05). 结论日本血吸虫感染小鼠伴随免疫新模型与血吸虫常规感染小鼠的细胞免疫功能变化趋势基本一致,表现为感染早期以Th1细胞应答为主,然后逐渐向Th2细胞极化.Objective On the base of the establishment of the new concomitant immunity mouse model infected with Schistosoma japonicum, to discuss the cell immunity mechanisms of this new model by observing the dynamic changes of Thl cytokine （IFN-γ） levels and Th2 eytokine （IL-4） levels in spleen cells stimulated by different antigens. Methods Each mouse was infected with 20 ±1 eercariea of S. japonicum. 20 days after infection, the mice of experimental group were injected with allyl thiourea at the dose of 300 mg/kg every day by intraperitoneal injection. At 0, 3, 6, 9 and 12 weeks after infection, the ELISA was used for the quantitative determination of IFN-γ and IL-4 produced by cultivated spleen cells in vitro after stimulated by soluble worm antigen preparation （SWAP）, soluble egg antigen （SEA） and Coneanavalin A （ConA）. Results The varianee tendeney of IFN-γ and IL-4 in the rou tine model and the new model of mice infected with S. japonicurn were generally eoineident, which showed that the levels of IFN-γ increased at 4-6 weeks after infection, arrived at peak at 7--9 weeks, and then decreased gradually at 10--12 weeks towards the levels of pre infection; and the levels of IL-4 began increasing gradually at 6 week after infection and continually maintained a high level until 12 weeks. The levels of IFN-γ stimulated by SEA and the levels of IL-4 stimulated by SWAP and SEA in the routine rood el groups were both obviously higher than that in the new model groups （P〈0.05）, while the levels of IFN-γ stimulated by SWAP in the new model groups were significantly higher than that in the routine model groups （P〈0.05）. In addition, the levels of IFN- γ and IL-4 stimulated by ConA were obviously higher than that stimulated by SWAP and SEA in all groups （P〈 0.05）. Conclusion The variance tendeney of cellular immune function of both new eoncomitant immunity mouse model and routine infectious model infected with S. japonicurn was generally eoineident.

关 键 词：血吸虫 日本 细胞因子 Γ干扰素 白细胞介素-4 

分 类 号：R383.24[医药卫生—医学寄生虫学]