热硼酸盐法提取梨雌蕊RNA方法改进及提取效果  

Study on Using Hot-borate Method in Extracting the Total RNA from the Pistil of Pyrus pyrifolia

作　　者：杨谷良[1] 谭晓风[2] 

机构地区：[1]黄冈师范学院生物系,湖北黄冈438000 [2]中南林学院经济林育种与栽培国家林业局重点实验室,湖南长沙410006

出　　处：《西北林学院学报》2006年第2期54-56,共3页Journal of Northwest Forestry University

基　　金：湖南省科技三项基金"湖南省主要沙梨品种自交不亲和基因的研究"(0JZY2155)

摘　　要：提取梨雌蕊的总RNA;方法:修改后的热硼酸盐法。采用电泳检测RNA的完整性、并测定了提取物的浓度和纯度、根据S基因设计引物对提取的总RNA进行3'-RACE实验等方法对提取产物进行检测。结果:修改后的整个提取过程可以在1 d内完成,提取的总RNA电泳条带清晰,总RNA提取液的浓度为1.925μg/L,OD260/OD280值为1.926,3'-RACE实验得到了预期的条带。结论:经过改进后的热硼酸盐法适合提取梨雌蕊的RNA。Objective. To extract the total RNA from the pistil of Pyrus pyrifolia; an improved hot-borate method was used. The integration of the RNA was ascertained by gel electrophoresis; the purity and the concentration was measured, and the test of 3＇-RACE was completed after the primer of P1 was designed based on the S gene. The results showed that the whole extraction process could be completed in 1 day. The strings of the gel electrophoresis of total RNA were distinctly. The concentration of the extracted RNA liquor was 1. 925μg/L. The value of OD260/OD280 was 1. 926 and the expected gel electrophoresis strings from 3＇-RACE emerged. It is suggested the improved hot-borate method is suitable to extract the total RNA from the pistil of P. pyrifolia.

关 键 词：热硼酸盐法 RNA提取 梨 雌蕊 

分 类 号：S718.43[农业科学—林学]