一株细菌儿茶酚型铁载体分泌的影响因素研究  

Study on Effect Factor of the Production of Catechol-type Siderophores Secreted by a Strain of Soil Bacteria

作　　者：谢小军[1] 王敬国[1] 

机构地区：[1]中国农业大学资源与环境学院农业部植物营养学重点实验室,北京100094

出　　处：《微生物学通报》2006年第1期105-109,共5页Microbiology China

基　　金：国家自然科学基金资助项目(No.30270768)~~

摘　　要：采用两种新的高分辨率的薄层层析(TLC)方法对一株土壤细菌S1在3种不同培养基上产生的儿茶酚型铁载体进行了分析。结果表明:不同培养基对铁载体的产生影响较大,在3种不同的培养基上菌株S1产生不同的儿茶酚铁载体,其中仅在1种培养基上S1能够分泌2,3-二羟基苯甲酸(2,3-DHBA)。同时,还分析了A l3+对S1分泌的儿茶酚型铁载体总量的影响,结果表明:A l3+能显著刺激铁载体的分泌,并且能抵消一定浓度范围内的Fe2+对铁载体分泌的抑制作用,KMB培养液中产生的4种儿茶酚铁载体中有3种和A l3+有较强的螯合力。Catechol-type siderophores secreted by a strain of soil bacteria in three different medium were assayed by two high-resolution TLC. The results showed different medium had a significant effect on the secretion of catechol-type siderophores, and in three different medium strain SI produced different catechol type siderophores. The effect of Al^3＋ on Catechol-typc siderophores by S1 were also assayed. The results showed Al^3＋ had a significant stimulation on the secretion of catechol-type siderophores. Moreover, Al^3＋ could to some extent counteract the repression of Fe^2＋ on siderophores production. In KMB medium four catechol-type siderophores were identified and all ones except for 2, 3 dihydroxybenzoic acid （2, 3-DHBA） had high affinity for Al^3＋.

关 键 词：土壤细菌 儿茶酚型铁载体 2 3-二羟基苯甲酸(2 3-DHBA) AL^3+ 

分 类 号：Q935[生物学—微生物学]