西南区多花黑麦草品种同工酶指纹图谱的构建及遗传背景分析  

Isozyme Fingerprint of Six Varieties of Lolium multifolium in South-west China and Their Genetic Background

作　　者：黄琳凯[1] 张新全[1] 马啸[1] 刘明秀[1] 曾琨[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014

出　　处：《安徽农业科学》2006年第3期426-427,443,共3页Journal of Anhui Agricultural Sciences

基　　金：四川省"十五"牧草育种攻关项目(02SG023-001);科技部星火计划项目(2004EA810010)资助

摘　　要：采用聚丙烯酰胺凝胶电泳的方法，对西南区主栽6个多花黑麦草品种进行了酯酶（EST）和过氧化物酶（POD）同工酶检测。通过酯酶检测到5种EST酶谱谱型，可以把4个品种区分开。过氧化物酶检测到4种POD酶谱谱型，可以把3个品种区分开。综合以上2种酶谱谱型可以鉴定供试的6个品种。6个多花黑麦草之间的相似系数0．615～0．941，品种间平均相似系数为0．7388。The esterase（EST） isozyme and peroxidase（POD） isozyme of Annual ryegrass in south-west China has been studied with polyareylamide gel eleetrophoresis, EST yielded 5 different isozume phenotypes and five varieties were discrinfinated by this enzyme alone. POD produced 4 different isozyme phenotypes and 4 varieties were separated. When both enzyme systems were taken together, 6 varieties were uniquely discriminated.The similarity coefficient among 6 varieties of annual ryegrass ratrged from 0.615 to 0.941 ,with an average of 0.738 8.

关 键 词：多花黑麦草 同工酶 品种鉴定 聚类分析 

分 类 号：Q55[生物学—生物化学]