一氧化氮对自发性高血压大鼠血管平滑肌细胞硫化氢/胱硫醚-γ-裂解酶的调节作用  

Regulatory Effect of Nitric Oxide on Hydrogen Sulfide/ Cystathionine-γ-Lyase of Vascular Smooth Muscle Cells in Spontaneously Hypertensive Rats

作　　者：金红芳[1] 杜军保[1] 唐朝枢[2] 卜定芳[2] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学第一医院心血管研究所教育部分子心血管病学重点实验室,北京100034

出　　处：《实用儿科临床杂志》2006年第3期140-141,共2页Journal of Applied Clinical Pediatrics

基　　金：国家杰出青年科学基金项目资助(30425010);国家重点基础研究发展规划项目资助(G2000056905)

摘　　要：目的研究一氧化氮(NO)对自发性高血压大鼠(SHR)血管平滑肌细胞硫化氢(H2S)/胱硫醚-γ-裂解酶(CSE)的调节作用。方法大鼠主动脉平滑肌细胞分为硝普钠(SNP)实验组和对照组,硫电极法测定培养上清中H2S含量,竞争性RT-PCR法检测细胞中CSE mRNA水平。结果SNP实验组较对照组培养上清H2S含量显著升高[(16.16±3.71)μmol/Lvs(22.71±4.84)μmol/L],但CSE mRNA的水平在SNP组较对照组明显降低[(0.16±0.06)vs(0.12±0.04)]。结论SNP对SHR血管平滑肌细胞中H2S/CSE体系发挥复杂的调节作用。Objective To investigate the effect of nitric oxide on hydrogen sulfide/cystathionin-γ-lyase（CSE） system in the vascular smooth muscle cells of spontaneously hypertensive rats（SHR）. Methods The SHR aortic smooth muscle cells （SMCs） were divided into 2 groups： sodium nitroprusside （SNP） group and control group. The content of hydrogen sulfide in the culture media was measured by sulfide electrode method, and the CSE mRNA level was assayed by competitive reversed transcription - polymerase chain reaction （RT- PCR）. Results Compared with control group, the content of hydrogen sulfide in the culture media of SNP group was significantly higher [ （16.16 ± 3.71 ） μmol/L vs （22.71 ± 4.84） μmol/L], and the CASE mRNA level in SMCs in SNP group was lower than that of control group. Concluslon Nitric oxide exerted complicated effect on the hydrogen sulfide/CSE system in the SHR smooth muscle cells.

关 键 词：一氧化氮 硫化氢 胱硫醚-Γ-裂解酶 高血压 平滑肌细胞 

分 类 号：R544.1[医药卫生—心血管疾病]