万寿菊的组织培养和瓶苗开花研究  

Rapid micro-propagation and inflorescence induction of Tagetes erecta L.

作　　者：邹永梅[1] 黄雪芳[1] 

机构地区：[1]江苏教育学学院生物系,江苏南京210013

出　　处：《江苏林业科技》2005年第6期17-19,共3页Journal of Jiangsu Forestry Science & Technology

摘　　要：对矮生杂交万寿菊组织培养研究表明:带1个腋芽的无菌苗茎段在MS+0.05 mg/L NAA+0.2 mg/L BA培养基上35 d均可诱导出芽,芽诱导率达100%;继代培养时,MS+0.1 mg/L BA是万寿菊较适宜的增殖培养基,平均增殖系数可达10.7,生根率为100%。将带有2个腋芽和1个顶芽的茎段接种到MS+0.05 mg/L BA+0.4 mg/LPP333培养基上,20 d后,就能长成1株有根、茎、叶和花蕾、花朵的完整植株。The results of tissue cuhure, rapid propagation and inflorescence inducement on Tagetes erecta L. were reported. Buds were induced from the segments cultured on MS medium supplemented with 0. 2mg/L BA and 0. 1mg/L NAA for 35 days, with an induction rate 100%. MS ＋0. 1 mg/L BA was a best media for multiplication, the average propagation coefficient was 10. 7, and the rooting rate 100%. After having cultured on MS medium supplemented with 0. 05 mg/L BA and 0. 4mg/L PP333 for 35 days, shoots became with infiorescences, buds and roots. The above-mentioned efficient and rapid production system has been successfully developed to propagate Tagetes erecta L.

关 键 词：万寿菊 微繁 芽诱导 增殖 成花诱导 

分 类 号：S682.1[农业科学—观赏园艺]