芸香苷对急性胰腺炎大鼠磷脂酶A_2和Ca^(2+)浓度的影响  

Effect of rutoside on phospholipase A_2 and Ca^(2+) in murine acute pancreatitis

作　　者：田洁[1] 宋少刚[1] 王宇翎[1] 陈志武[1] 赵维中[1] 

机构地区：[1]安徽医科大学药理教研室,安徽合肥230032

出　　处：《安徽医药》2006年第2期88-90,共3页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省科技厅自然科学基金(编号:01043907)

摘　　要：目的探讨芸香苷(Rutoside,Ru)对急性胰腺炎(AP)大鼠磷脂酶A2(PLA2)和Ca2+浓度的影响。方法脱氧胆酸钠逆行胆胰管注射和胆胰管结扎诱发大鼠AP模型。检测造模后6、12 h血清中Ca2+含量和造模后24 h胰腺组织中PLA2和Ca2+含量。结果Ru 60、120 mg.kg-1(sc)可明显升高造模后12 h AP大鼠的血清Ca2+浓度。Ru(30、60、120 mg.kg-1,sc)可明显增加胰腺组织降低的PLA2含量,降低模型大鼠的胰腺组织过高的Ca2+水平。结论Ru提高胰腺组织PLA2含量,提示Ru可能对胰腺组织PLA2的释放及激活具有抑制作用;Ru能有效防止AP大鼠低钙血症的发生,并降低胰腺组织细胞Ca2+超载。Aim To study the effect of rutoside （Ru） on phospholipase A2 and Ca^2＋ in murine acute pancreatitis AP. Methods Acute pancreatitis （AP） model was induced by retrograde injection of sodium deoxycholate into bilipancreatic duct or by ligating the biliopan- creatic duct at the point of entry into the duodeum in rats. Ca^2＋ concentrations of serum and pancreas tissue and PLA2contents of pancreas tissue in rats were measured. Results Ru（60,120 mg· kg^-1,sc） increased serum Ca^2＋ concentrations after the induction of AP at 6,12 h. Ru（30,60,120 mg · kg^ - l, sc） , decreased CaCa^2＋ concentrations of pancreas tissue and raised PLA2 levels after the induction of AP at 24 h significantly. Conclusion The study indicated that the mechanisms of protective effect of Ru on the experimental AP might block the translocation of calcium from extracellular to intracellular compartments and attenuate the injury of Ca^2＋ overload in acinus. Maybe, the antagonistic effect is also related to the inhibition on PLA2activation.

关 键 词：芸香苷 急性胰腺炎 血清淀粉酶 磷脂酶A2 CA^2+ 

分 类 号：R285[医药卫生—中药学]