高效液相色谱法测定人血浆中盐酸伪麻黄碱浓度  

Determination of pseudoephedrine hydrochloride in human plasma by HPLC

作　　者：张善堂[1] 舒冰[1] 史天陆[1] 沈爱宗[1] 屈建[1] 

机构地区：[1]安徽省立医院,安徽合肥230001

出　　处：《安徽医药》2006年第2期110-111,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立测定人血浆中盐酸伪麻黄碱浓度的方法。方法血浆样品经混合碱液碱化、甲基叔丁基醚萃取、0．5％盐酸溶液酸化反提处理后，采用反相HPLC法测定。色谱柱：Kromasil C18柱（250mm×4．6mm，5μm），柱温30℃；流动相：0．01mol·L^-1磷酸盐缓冲液（pH6．5）-乙腈（62：38），流速1．0ml·min^-1；检测波长210nm。结果盐酸伪麻黄碱在0．015—1．60mg·L^-1范围内线性关系良好，回归方程为：Y=4631．8X＋3．76（r=0．9998），该方法平均回收率为101．7％，日内RSD为3．41％-4．25％，日问RSD为6．47—9．66％。结论该法简便、灵敏、快速、稳定性好，可用于临床血药浓度检测及药代动力学研究。Aim To establish a senfive and specific method for determination of pseudoephcdrine hydrochloride in human plasma. Methods Pseudoephedrine was extracted from alkaline plasma with t-butyl methyl ether as the base form,and was back-extracted into 0.5% hydrochloride solution. The drug was determined by RP-HPLC. The LC-6A HPLC apparatus and Kromasil C18 column were used with mobile phase of 0.01 mol· L^-1 KH2 PO4 （pH6.5）-acetonltrile （62：38 ） ,The flow rate was 1.0ml · min^- 1 and the detection wavelength was 210 nm. Results The linear range of pseudoephedrine was 0.015 -1.60 mg·L^-1 ,（Y =4631.8X ＋3.76,r =0. 999 8） ,The average recovery of pseudoephedrine in human plasma was 101.7%. The within-day RSD were 3.41% -4.25% ,and the daytime RSD were 6. 47% - 9.66%. Conclusion The method was specific, sensitivity, simple and suitable for the determination of human plasma pseudoephedrine and pharmacokinefic researches.

关 键 词：盐酸伪麻黄碱 血药浓度 反相高效液相色谱法 

分 类 号：R96[医药卫生—药理学]