“夏光”、“早夏16”甘蓝杂交种DNA指纹图谱的构建  

Establishment of DNA fingerprints on two cabbage hybrids of "Xiaguang" and "Zaoxia 16"

作　　者：刘冲[1] 王曙霞[1] 任云英[1] 陈锦秀[1] 葛才林[2] 薄天岳[1] 

机构地区：[1]上海市农业科学院园艺研究所上海市设施园艺技术重点实验室,上海201106 [2]扬州大学农学院,扬州225009

出　　处：《上海农业学报》2006年第1期31-33,共3页Acta Agriculturae Shanghai

基　　金：上海市科委基础性研究计划项目(03JC14060);上海市农委科技兴农重点攻关项目[沪农科攻字(2004)第2-5号]资助

摘　　要：用SDS法提取甘蓝(Brassica oleraceavar.capitata)杂交种“夏光”、“早夏16”及其各自亲本的基因组DNA,通过SSR、RAPD两种分子标记方法构建其DNA指纹图谱用于纯度鉴定。利用30对甘蓝SSR引物和50个适用于甘蓝的RAPD引物,以各杂交种及其亲本的基因组DNA为模板组合进行筛选,结果显示:多数SSR引物对两组合扩增带型一致,未能建立SSR指纹图谱;通过RAPD标记方法筛选出能鉴定两杂交种纯度的引物分别为S15、S42、S147和S42、S78、S88,其中引物S42对两组合均能扩增出特异的RAPD指纹图谱,并将RAPD指纹图谱转变为相应的数字指纹。The genomic DNAs were extracted by the method of SDS from two cabbage （ Brassica oleracea vat, capitata） hybrids （＂Xiaguang＂, ＂Zaoxia 16＂） and their parents in order to establish their DNA fingerprints by RAPD and SSR methods for identification of seed purity, The genomic DNAs of each hybrid and its parents were used as one group of template DNA to screen 30 pairs of SSR primers and 50 RAPD primers. The results indicated that the types of DNA bands amplified by most SSR primers in the two groups was similar, so the establishment of SSR fingerprints failed, But 3 efficacious RAPD primers were gained in each group, which were S15, S42, S147 and S42, S78, S88 respectively. The specific RAPD fingerprints in the two groups could be established with S42 primer and then transformed into digital fingerprints.

关 键 词：甘蓝 杂交种 SSR RAPD DNA指纹图谱 

分 类 号：S635[农业科学—蔬菜学]