水溶性醇类对溶菌酶空间结构和折叠过程的影响  

Effect of soluble alcohols on conformational changes and refolding of lysozyme

作　　者：王玮[1] 白姝[1] 董晓燕[1] 孙彦[1] 

机构地区：[1]天津大学化工学院生物化工系,天津300072

出　　处：《化工学报》2006年第1期74-78,共5页CIESC Journal

基　　金：国家自然科学基金项目(20476081).~~

摘　　要：利用多角度激光光散射仪和圆二色谱仪研究小分子水溶性一元醇和多元醇对溶菌酶分子的稳定性以及对变性溶菌酶的空间构象和折叠复性的影响.发现在一元醇和二元醇溶液中溶菌酶分子的稳定性均随着醇浓度的增大而提高,而变性酶分子在醇溶液中形成过多的α螺旋和β折叠等二级结构不利于酶的折叠复性;只有较低浓度的多元醇和甲醇有辅助变性溶菌酶复性的作用,其中10%甲醇、20%甘油和30%乙二醇有利于促进酶的复性.The effects of alcohols on the intermoleeular interactions of lysozyme changes and refolding pathway of denatured lysozyme were studied by using laser 1 diehroism spectroscopy and activity assay. The results indicated the presence o and the conformational ight scattering, circu f strong intermolecu ar ar repulsion or weak attraction between lysozyme molecules at low alcohol concentrations. The eonformational changes induced in the presence of alcohol did not promote folding to its native structure. At low concentrations of alcohols such as 20% glycerol, 30% glycol and 10% methanol, the denatured lysozyme could be refolded with high activity yields.

关 键 词：醇 溶菌酶 复性 分子相互作用 空间结构 

分 类 号：O629.73[理学—有机化学] O643[理学—化学]