离子辐照大豆子叶细胞显微分析  

MICROSCOPIC ANALYSIS OF THE CELLS OF SOYBEAN COTYLEDON BY LON RADIATION

作　　者：余增亮[1] 邓建国[1] 吴跃进[1] 何建军[1] 尹载群 

机构地区：[1]中国科学院等离子体物理研究所

出　　处：《安徽农业科学》1990年第1期27-30,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金

摘　　要：离子束辐照大豆子叶细胞表面会产生溅射现象.来自子叶的具有能量和方向的溅射粒子便沉积在载体上并形成生物薄膜.用辐照样品在扫描电镜下观察时,可发现子叶表面有辐照损伤和离子刻蚀.对不同组织,例如细胞壁、细胞质、胞间层等的刻蚀程度,常因它们的溅射速度而异.用400倍显微镜观察薄膜上的少许小薄片时,可见由溅射现象形成的生物薄膜展现光滑、淡黄并具强附着力的特性.曾用生物学和化学方法分析得知,薄膜的成分主要由蛋白质和脂肪组成.从而可以认为,其成分基本上与大豆子叶的成分相吻合.The sputter was occured when ion beam had radiated on the surface of a soybean cotyledon. The sputtering particles from the cotyledon with energies and direction could deposit on a substrate, and biological film was produced. There were the radiant damage and the ionic etching on the surface of the cotyledon when the radiant sample was observed by using a scanning electron microscope(SEM). For differe- rent tissues such as the cell wall, protoplasm, middle lamella, the etching degree was different because of their sputtering ratio. The bi- ological film was formed by the sputter but exhibited smooth, light ye- llow and strong adhesion. When a little small slices of the cells on the film was observed by×400 microscope. The components of the film had been analyzed by biological-chemical methods and consists m- ainly of protein and fat. It would be seen from that the components of film were basically consistent with that of the cotyledon.

关 键 词：大豆 大豆子叶 离子辐照 显微分析 

分 类 号：S565.101[农业科学—作物学]