板蓝根高极性部位增强F_(022)抗内毒素活性的研究  

Top-polar Fraction Developing Anti-endotoxin Activity of F_(022) from Radix Isatidis

作　　者：林爱华 刘奕明 丘小惠 刘云海[2] 

机构地区：[1]广州中医药大学第二附属医院,广东广州510120 [2]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《时珍国医国药》2006年第2期156-157,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(No.39870872)

摘　　要：目的研究板蓝根高极性部位(Ftp)与板蓝根F022部位协同抗内毒素作用。方法ELISA法检测内毒素刺激鼠腹腔巨噬细胞分泌肿瘤坏死因子α(TNF-α)、白介素6(IL-6)水平,内毒素致BCG增敏小鼠死亡实验。结果Ftp联合F022处理巨噬细胞,能显著抑制TNF-α和IL-6水平,效果优于单用F022组;Ftp联用F022显著延长BCG增敏小鼠受内毒素攻击后的存活时间。结论Ftp本身抗内毒素活性较弱,但与F022部位合用,可进一步增强F022部位的抗内毒素作用。Objective To study the anti - endotoxin synergistic effect of top - polar fraction （ Ftp ） and F022 from Radix Isatidis. Methods The production of TNF -α and IL - 6 of murine peritoneal macrophages stimulated by LPS was measured by ELISA. The lethality of BCG -primed mice was induced by LPS. Results Ftp and Fo22was added simultaneously to macrophages culture lh before addition of LPS, Production was remarkably inhibited. F,pand F022 remarkably protected BCG - primed mice from LPS induced lethality. Conclusion These results suggest that Ftp can develop the anti - endotoxin activity of F022 from Radix Isatidis.

关 键 词：板蓝根 抗内毒素活性 TNF-Α IL-6 

分 类 号：R285.6[医药卫生—中药学]