败酱草多糖体外抗呼吸道合胞病毒作用的研究  

In vitro anti-respiratory syncytial virus effect of an active compound (AP4) from Herba patriniae

作　　者：张凤梅[1] 李洪源[1] 李霞[1] 刘鑫妍[1] 王秀琴[1] 陈振华[1] 谢志平[1] 李鑫[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院流行病学教研室,黑龙江哈尔滨150086

出　　处：《黑龙江医药科学》2006年第1期48-50,共3页Heilongjiang Medicine and Pharmacy

基　　金：哈尔滨市科委攻关课题项目编号:2005AA3CS168

摘　　要：目的:了解败酱草多糖(AP4)对呼吸道合胞病毒的体外抑制作用。方法:经提取、沉淀、离心、大孔吸附树脂两次层析后,得败酱草抗病毒多糖AP4。通过细胞培养法检测呼吸道合胞病毒对Hela细胞的致病变作用及AP4对呼吸道合胞病毒感染Hela细胞的治疗作用。结果:AP4半数中毒浓度(TC50)为11.07mg/ml,抑制呼吸道合胞病毒的半数有效浓度(EC50)为0.097mg/ml,治疗指数(TI)为114;病毒唑半数中毒浓度(TC50)2.087mg/ml,抑制呼吸道合胞病毒的半数有效浓度(EC50)为0.0385mg/ml,治疗指数(TI)为54。结论:败酱草抗病毒有效部位AP4在Hela细胞中对呼吸道合胞病毒有明显的抑制作用。Objective： To investigate the in vitro inhibition of respiratory syncytial virus by an active compound （AP4） from a Chinese medicine herb--Herba patriniae. Methods： Active component of herba patriniae （AP4） was extracted and its anti-- respiralory syncytial virus effect was tested . A water soluble substance （AP4） was isolated from Herba patriniac, by hol water extraction ,ethanol precipitation and macroporous adsorptive resins adsorption. Chemical tests showed that the substance was polysaccharide, AP4 was active against the respiratory syncytial virus by CPEI assay while Ribavirin was used as positive control . Results： Chemical tests showed that the substance was polysaccharide. The median toxic eoncentration（TC50） of AP4 was 11.07 mg/ml by morphological observation under light microscope , the medium effective concentration（EC50） against replication of lhe long strain of respiratory syncytial virus in Hela cells was 0. 097 mg/ml and therapeutic index（TI=TC50/ EC50） of AP4 was 114. The median toxic concentratlon（TC50） of ribavirin was 2. 087 mg/ml, its medium effective concentration （EC50） was 0. 0385mg/ml and therapeutic index （TI= TC50/ EC50） of Ribavirin was 54. Conclusion： AP4 has an obvious inhibitor effect on RSV in Hela cell culture .

关 键 词：败酱草多糖 呼吸道合胞病毒 抗病毒作用 

分 类 号：R978.7[医药卫生—药品]