乙型肝炎病毒感染者HBV DNA与血清病毒标志物的关系  

Relationships among HBV DNA and HBV immunological markers in patients with chronic hepatitis type B

作　　者：范火亮 

机构地区：[1]惠州中心医院检验科,广东省惠州516001

出　　处：《中国基层医药》2006年第1期64-65,共2页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨乙型肝炎病毒(HBV)感染者HBV DNA检出情况及其与血清病毒标志物的关系。方法采用实时荧光定量聚合酶链反应(FQ-PCR)检测200份HBV感染者血清中HBV DNA含量,同时用全自动免疫分析仪检测HBV5项血清标志物。结果200份血清中,乙型肝炎病毒标志物不同组合组的HBVDNA阳性率有差异,HBsAg、HBeAg和(或)HBcAb阳性组阳性率为98.1%,其中96%以上HBV DNA≥104cope/ml;HBsAg、HBcAb和(或)HBeAb阳性组HBV DNA阳性率约为76.5%,但其中90%以上HBV DNA≤104cope/ml;单纯HBV抗体阳性组HBV DNA阳性率约为13.0%,但均为HBV DNA≤103cope/ml。三组间HBV DNA阳性率及分布均有明显差异(P<0.05)。而且HBV DNA含量与疾病严重程度相关。结论FQ-PCR检测HBV DNA含量结合血清病毒标志物的检测结果对乙型肝炎的临床诊断与病情判断有重要意义。Objective To investigate the relationship among HBV DNA and HBV immundogical markers in patients with chronic hepatitis type B. Methods The amount of HBV DNA in the serum of 200 patients were estimated by FQ -PCR. The serum HBV immunological markers of these patients were tested with ELISA. Results The amount of HBV DNA was different in 3 HBV immunological markers groups. The amount of HBV DNA in HBsAg, HBeAg and（or） HBeAb positive samples was higher than that in other groups with positive rate of 98.1% . The HBV DNA positive raw of HBsAg, HBeAg and（or） HBeAb positive samples was 76.5 % ;The positive rate of HBcAb positive samples was 65 % ; The positive rate of HBcAb/HBeAb/HBsAb positive samples was 13 %, but all of them,HBV DNA≤10^3cope/ml. Each group were difference in HBV DNA result. Conclusion FQ-PCR has high sensitivity and specificity. The amount of HBV DNA with HBV immunological markers has important signification in diagnosis and judgement of patients with chronic hepatitis type B.

关 键 词：HBV DNA 聚合酶链反应 生物学标记 乙型肝炎病毒感染 血清病毒标志物 

分 类 号：R512.62[医药卫生—内科学]