雏鸡感染毒害艾美耳球虫后免疫器官T细胞亚群的动态变化  

Dynamic Changes of T Lymphocyte Subpopulations in Immune Organs of Chicks Infected with Eimeria necatrix

作　　者：刘晶[1] 郑世民[1] 胡京友[2] 

机构地区：[1]东北农业大学动物医学学院 [2]增城出入境检验检疫局,增城511340

出　　处：《畜牧兽医学报》2006年第2期168-172,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：黑龙江省自然科学基金项目(C0206)

摘　　要：采用ABC法检测了毒害艾美耳球虫（Eimeria necatrix）初次、二次感染雏鸡后，其免疫器官T细胞亚群的动态变化。结果发现：雏鸡初次感染E．necatrix后，其胸腺和脾脏的CD4^＋T细胞和CD8^＋T细胞于感染后4～21d不同程度高于未感染的对照雏鸡，14～16d达峰值，随后缓慢下降。其中CD4^＋T细胞较CD8^＋T细胞增殖辐度大，持续时间也较长。Enecatrix二次感染雏鸡后，上述免疫器官的CD4^＋T细胞和CD8^＋T细胞数量在感染后2～7d呈下降趋势，随后回升，至10～14d明显高于对照和初次感染雏鸡。其中CD8^＋T细胞在二次感染后降幅较小，回升更迅速，增殖幅度也较大。表明鸡CD4^＋T细胞和CD8^＋T细胞在抵抗E．necatrix感染的不同时期发挥不同的作用。Avidin-biotin complex （ABC） methed was used to study the dynamic changes of T lymphocyte subpopulations in immune organs of chicks after primary and secondary infection with E. necatrix. The results were as follows. The numbers of CD4^＋ and CD8^＋ ceils in thymus and spleen of chicks after primary infection with E. necatrix were increased comparing with controls 4 -21 days post infection （PI） to some extent. The number increased significantly to a peak around 14-16 days PI. Compared with CD8^＋ T cells, CD4^＋ T cells increased rapidly and continued long period. The numbers of CD4^＋ and CD8^＋ T cells in immune organs of chicks after secondary infection with E. necatrix were decreased 2-7 days PI to some extent. Thereafter they begin to increase. The number increased significantly comparing with controls and primary infection chicks at 10-14 days PI. The CD8^＋ T cell decreased slightly and increased rapidly than CD4^＋ T cells. The results indicated that CD4^＋ and CD8^＋ T cells play variously role in different period when chicks resisted E. necatrix infection.

关 键 词：雏鸡 毒害艾美耳球虫 免疫器官CD4^+T细胞 CD8^+T细胞 

分 类 号：S855.9[农业科学—临床兽医学]