不同理化因子对甘草愈伤组织生长和黄酮类化合物合成的影响  

Effects of Physical and Chemical Factors on Callus Growth and Flavonoid Production in Glycyrrhiza uralensis Calli

作　　者：杨世海[1] 刘晓峰[2] 马秀华[3] 果德安[4] 郑俊华[4] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林亚泰(集团)股份有限公司,长春130031 [3]吉林农业大学图书馆,长春130118 [4]北京大学药学院,北京100083

出　　处：《吉林农业大学学报》2006年第1期47-50,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(39470849)

摘　　要：试验研究了不同理化因子对甘草愈伤组织生长和甘草素、异甘草素、甘草甙、异甘草甙和甘草查尔酮等5种甘草黄酮类化合物合成的影响。结果表明：果糖有利于甘草愈伤组织生物量积累和黄酮类化合物生物合成。以2％果糖作为碳源时，培养物中5种黄酮类化合物的含量是蔗糖作为碳源时的2倍，在2％～6％范围内，随着果糖浓度的增加，培养物中5种黄酮类化合物的含量呈下降趋势，其中甘草查尔酮的含量增加5倍以上。光照对愈伤组织生长影响不大，但能促进黄酮类化合物的生物合成，光培养下黄酮类化合物的含量是黑暗条件下的2倍。甘草愈伤组织生长及黄酮类化合物生物合成的适宜温度约为25℃，当培养温度低于15℃或高于30℃时，愈伤组织生长及黄酮类化合物形成均受到抑制。The effects of physical and chemical factors on callus growth and flavonoid production in Glycyrrhiza uralensis calli were studied. The results showed that fructose was better than sucrose for callus growth and flavonoid formation, and the optimum concentration was 2%. The flavonoids content was 2 times higher than that of sucrose as carbon sources. In the range of 2%～6% fructose concentration, the flavonoids content decreased with the increase of the concentration, but the licochalcone increased 5-fold. Light had no significant effect on callus growth, but enhanced the flavonoids production. The flavonoids content in light was 2 times higher than that of cultures in dark. The optimum temperature for callus growth and flavonoids production was 25℃. The callus growth and flavonoids formation were inhibited at 15℃ and 30℃.

关 键 词：甘草 愈伤组织 理化因子 黄酮类化合物 

分 类 号：S567.1[农业科学—中草药栽培]