区带毛细管电泳分离测定大黄提取液中游离蒽醌化合物  

Separation and Determination of Anthraquinone Compounds in Chinese Herb Rhubarb Extracts by Capillary Zone Electrophoresis

作　　者：李伟[1] 柴金玲[1] 阿里木江.艾拜都拉 谷学新[1] 

机构地区：[1]首都师范大学化学系,北京100037

出　　处：《分析科学学报》2006年第1期68-70,共3页Journal of Analytical Science

摘　　要：采用区带毛细管电泳法分离和同时测定大黄提取液中五种蒽醌化合物的含量。毛细管电泳的分离条件为:50 cm×75μm i.d.未涂层石英分光毛细管,分离电压20kV,0.05 mol/L KH2PO4-Na2HPO4(pH=8.2)缓冲溶液,紫外检测波长为254 nm,以氨基苯磺酸为内标。该法简便,快速,分辨率高,重现性好。A capillary zone electrophoresis method has been developed for the simultaneous determination of five anthraquinones in rhubarb extracts. The separation was performed on an uncoated fused silica capillary of 50 cm×75μm i.d. at an applied voltage of 20 kV with 0.05 mol/L phosphate （pH=8.2） buffer solution. The anthraquinones were detected at wavelength of 254 nm. Sulfanilic acid was used as the internal standard. The result showed that the method was accurate, simple, rapid and reproducible.

关 键 词：毛细管电泳 大黄 芦荟大黄素 大黄素 大黄素甲醚 大黄酚 大黄酸 

分 类 号：O657.8[理学—分析化学]