紫外分光光度法测定灵芝及调脂灵中总灵芝酸的含量  

Quantification of Ganoderic Acids in Ganoderma Lucidum and TiaoZhiling by Ultraviolet Spectrometry

作　　者：吴禾[1] 刘晔[2] 姜华 

机构地区：[1]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071 [2]中国人民解放军军事医学科学院放射与辐射医学研究所,北京100850 [3]北京市扬格保健品公司,北京100025

出　　处：《解放军药学学报》2006年第1期74-76,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的 建立灵芝和调脂灵中总灵芝酸的含量测定方法。方法 采用紫外分光光度法，以灵芝酸C2为对照品，饱和NaHCO3溶液为溶剂，测定波长260nm。结果 灵芝酸C2在0．0108-0．0486mg·ml^-1范围内呈良好的线性关系，r=0．9995；加样平均回收率为99．2％，RSD为1．76％（n=5）。结论 该法操作简便，灵敏度高，专属性好，重复性好，可以用于灵芝及其凋脂灵的质量控制。Aim An assay method was developed for determination of ganoderic acids in C.anoderma Lucidum and TiaoZhiling by Ultraviolet Spectrometry. Methods A ganoderic acid C2 standard dissolved in saturated NaHCO3 has a maximum absorbance at 260nm by UV spectrometry. Results The concentration of standard is correlated very well to its optical density at 260nm in the range of 0.010 8 - 0.048 6mg·ml^-1 with a relativity coefficient of 0.999 5.The average recovery was 99.2% （ RSD = 1.76%, n = 5）. Conclusion The above method was easy to use, highly accumt, specific and repetitive, and it should be helpful for monitoring the quality of Ganoderma lucidum material and related products.

关 键 词：灵芝 调脂灵 灵芝酸 紫外分光光度法 

分 类 号：R917[医药卫生—药物分析学]