单克隆抗体标志乳胶凝集试验快速检测金黄色葡萄球菌及其耐药性  

Rapid Identification of Staphylococcus aureus and Its Resistance Against Meticillin Using Slidex-staph Monoclonal Antibody

作　　者：王崇义 俞晓春 徐晓 

机构地区：[1]浙江省宁海县第一医院,浙江宁海县315600 [2]浙江省医药高等专科学校,浙江宁波315000

出　　处：《中华医院感染学杂志》2006年第2期126-128,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨金黄色葡萄球菌(SAU)的细菌学快速检测鉴定及其耐药性分析的试验方法。方法目标菌落先经传统鉴别方法怀疑葡萄球菌,采用Slidex MRSA Detection乳胶快速检测SAU菌株青霉素结合蛋白2a(PBP2a),确认耐甲氧西林金黄色葡萄球菌(MRSA);设立SAU耐苯唑西林筛选试验对照,两组不符合菌株经PCR方法检测MceA基因确认。结果456株葡萄球菌中Slidex(staph.plus乳胶凝集法阳性(鉴定为SAU)135株,135株凝集法阳性菌中最后确认为SAU 127株,321株凝集法阴性菌最后确认为SAU11株,乳胶凝集法鉴定金黄色葡萄球菌灵敏度92.0%,特异性97.5%;Slidex MRSA Detection乳胶快速检测138株SA的PBP2a,阳性52株,138株SAU经苯唑西林筛选试验及PCR测定mceA基因,确定MRSA57株,乳胶凝集法快速检测SAU的PBP2a确定MRSA,灵敏度91.2%,特异性100%。结论单克隆抗体乳胶凝集法快速检测SAU及鉴定MRSA,具有较高灵敏度和特异性。OBJECTIVE To develop a rapid method for simultaneously identifying Staphylococcus aureus （SA） and its resistance against meticillin. METHODS The target strains authenicated to Staphylococcus by traditional methods （appearance,Gram stain,catalase） first, then using Slidex staph, plus to authenticate SA; establishing traditional method or coagulase test and ATB-IDS2STAPH system for verification. The penicillin-binding protein 2a（PBP2a） was examined by Slidex MRSA Detection, establishing resistance oxacillin sieving test and meeA gene was examined by PCR for verification. RESULTS The 135 strains were positive and 321 strains were negative for Slidex staph, plus from 456 strains. The 127 S. aureus strains and eight others were confirmed from 135 positive strains finally, 11 SA strains and 310 other strains were confirmed from 321 negative strains, there were 92.0% for sensitivity and 97.5% for specificity in this method. The 52 strains PBP2a positively confirmed to metieillin-resistant S. aureus（MRSA） using Slidex MRSA Detection and 57 MRSA strains were confirmed using resistance oxaciUin sieving test or PCR from 138 strains. There were 91.2% for sensitivity and 100% for speeifity in this method. CONCLUSIONS The duplex Slidex -staph monoclonal antibody examinated to SA and confirmed to MRSA has higher sensitivity and specificity.

关 键 词：金黄色葡萄球菌 耐甲氧西林金黄色葡萄球菌 mceA基因 聚合酶链反应 青霉素结合蛋白2a) 单克隆抗体 乳胶凝集法 

分 类 号：R378[医药卫生—病原生物学]