放线菌素X_2高产菌株的抗药性致死突变标志的诱变筛选研究  

A Study on UV Induced Screening of High Actinomycin X_2 Producing Strain by Lincomycin-resistant Lethal Mutation

作　　者：徐平[1] 熊智强[1] 涂国全[1] 

机构地区：[1]江西农业大学生物工程系,江西南昌330045

出　　处：《江西农业大学学报》2006年第1期122-125,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：教育部重点科技项目(2003-03072);江西省自然科学基金项目(0530010)

摘　　要：链霉菌702菌株所产抗细菌活性物质为放线菌素X2,为了提高其发酵单位,试验采用了林可霉素对放线菌素X2产生菌———链霉菌702孢子致死标志的UV诱变筛选。试验结果表明,紫外作用20 s时链霉菌702林可霉素致死标志的突变率高达20.77%,抗药性突变菌株与产放线菌素X2高产菌株的正突变率达25%,效价提高20%以上达10%,从突变菌株中摇瓶筛选到42-3473菌株,摇瓶发酵生物效价达2 062μg/mL,比初始菌株生物效价提高了70%。The antibacterial compound produced by Streptomyces 702 was Actinomycin X2. In order to increase the yield of Actinomycin X2, UV induced - mutation and lincomycin - resistant method was used to screen high - yield mutants of Streptomyces 702. The results showed that the lincomycin - resistant mutational frequency of Streptomyces 702 was up to 20.77% after treated by UV with 20 seconds. The high - yield mutants were screened at a high frequency of 25%. Furthermore, the high - yield mutational frequency reached 10% , and the products of mutants were 20% higher than those of the original strains. The yield of mutant 42 -3473 was up to 2 062 μg/mL, which was 70% higher than that of the original strain.

关 键 词：链霉菌702 UV 放线菌素X2 抗药性致死突变 

分 类 号：Q936[生物学—微生物学]