尖孢镰刀菌中β-D-葡萄糖苷酶活性测定条件的研究  

Studies on Testing Conditions for β-D-Glucosidase Activity in Fusarium oxysporum

作　　者：车建美[1] 刘波[1] 朱育菁[1] 蓝江林[1] 林抗美[1] 肖荣凤[1] 

机构地区：[1]福建省农科院生物技术研究所,福建福州350002

出　　处：《江西农业大学学报》2006年第1期126-128,共3页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家863计划项目(2002AA244031-2);福建省科技厅重大项目(2000Z031);福建省计委项目(闽计农经[2002]48号)

摘　　要：试验以对硝基-β-D-葡萄糖苷为底物,对尖孢镰刀菌中的β-D-葡萄糖苷酶活性测定条件进行了研究,包括缓冲液pH值、底物浓度、反应温度和时间等条件对酶活性的影响。结果表明,当温度为50℃、pH5.0、底物浓度达到4 mmol/L、反应时间为16 m in时,酶活力达到最大。The conditions including substrate concentration, buffer pH, reaction temperature and time etc. for testing β - D - Glucosidase activity in Fusarium oxysporum using p - Nitrophanyl - β - D - Gluopyranoside （pNPG） as substrate were studied. The results showed that enzyme extracts from Fusarium oxysporum showed the highest absorbance when reacted with pNPG at the concentration of 4 mmol/L under the conditions of reaction temperature 50 ℃ , buffer pH5.0 and reaction time 16 min.

关 键 词：尖孢镰刀菌 β-D-葡萄糖苷酶 对硝基-β-D-葡萄糖苷 

分 类 号：Q344[生物学—遗传学]