凝结芽胞杆菌对小鼠免疫功能和粪便胺含量及肠道中氨含量的影响  

The effects of Bacillus coagulans on immune functions,amine content of feces and ammonia content of intestinal tract in mice

作　　者：王文杰[1] 刘洋[1] 彭珊瑛[1] 万阜昌 崔云龙 李雄彪 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050 [2]青岛东海药业有限公司,青岛266400 [3]青岛市微生态工程技术研究中心,青岛266400

出　　处：《中国微生态学杂志》2006年第1期6-8,共3页Chinese Journal of Microecology

基　　金：国家科技部创新基金项目(01C26211100765);国家科技部863计划项目(2003AA2Z3C50)

摘　　要：目的研究凝结芽胞杆菌对小鼠的免疫功能、粪便胺含量及肠道中氨含量的影响。方法采用碳粒廓清法、免疫器官指数和血清溶血素测定法,分别考察药物对正常小鼠、免疫低下小鼠的非特异性免疫功能、特异性体液免疫功能的影响。同时观测药物对小鼠的盲肠内容物的氨含量和粪便中胺含量的影响。结果凝结芽胞杆菌对正常小鼠碳粒廓清指数和脏器指数没有明显影响,左旋咪唑则能显著提高其吞噬指数但对脏器指数没有影响。凝结芽胞杆菌和左旋咪唑均能显著提高免疫功能低下小鼠血碳粒廓清指数和胸腺指数,增加血清溶血素的生成。服用凝结芽胞杆菌后的小鼠排出的粪便胺含量和肠道中氨含量,均比正常对照组显著降低。结论凝结芽胞杆菌能显著提高免疫功能低下小鼠的免疫功能,减少正常小鼠肠道中胺和氨的含量,增强机体抵抗力和保护机体。Objective To investigate the effects of Bacillus coagulans on immune functions,amine content of feces and ammonia content of intestinal tract in mice.Methods The clearance rate of charcoal particles,the weight of immune organs and hemolysin production were measured to study the effects of Bacillus coagulans and levamisole on nonspecific immunity,specific humoral immunity and immune organs in normal or immunosuppressive mice induced by hydrocortisone.Moreover,their influences on amine content of feces and ammonia content of intestinal tract in normal mice were determined.Results Levamisole could significantly increase the clearance rate of charcol particles but did not influence immune organ inedx in normal mice.Differently,Bacillus coagulans had little effect on the both indicators.However,they all could obviously elevate the clearance rate of charcoal particles and thymus organ index in immunosuppressivo mice and notably raise hemolysin production in serum of SRBC-immunized mice.After administration of Bacillus coagulans for 21 days,amine content of feces and ammonia content of intestinal tract in nice were significantly lower than that control.Conclusion Bacillus coagulans could enhance immune functions in immunosuppressive mice.and reduce the amine and ammonia content in intestinal tract in normal mice.

关 键 词：凝结芽胞杆菌 左评剧咪唑 免疫功能 胺和氨含量 

分 类 号：R378.6[医药卫生—病原生物学]