尼古丁猝灭牛血清蛋白荧光机制的研究  

Studies on the Quenching of Fluorescence of Bovine Serum Albumin by Nicotine

作　　者：薛泽春[1] 程新胜[1] 杨丽文[1] 张现[1] 席征[1] 

机构地区：[1]中国科学技术大学烟草与健康研究中心,合肥230052

出　　处：《上海交通大学学报（农业科学版）》2006年第1期44-47,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家烟草专卖局科学基金资助项目(No.110200302016)

摘　　要：研究了尼古丁对牛血清蛋白荧光光谱变化,以及牛血清蛋白荧光猝灭机制,同时利用同步荧光研究了尼古丁对酪氨酸和色氨酸的影响,结果表明:尼古丁猝灭牛血清蛋白荧光是通过静态机制,形成常数为KA=2.1×107 L.mol-1,每一个牛血清蛋白分子结合尼古丁的位点数为0.22,牛血清蛋白与尼古丁的这种结合是很弱的,没有影响分子的构象,酪氨酸残基荧光没有变化,只是色氨酸荧光强度降低,荧光峰位置不变。The fluorescence spectra was employed to study the interaction between nicotine and BSA, and we also studied the affecting of nicotine to Trp,Tyr residues by synchronous fluorescence .The results demonstrate that nicotine could quench the fluorescence of BSA by mechanism of static process rather than dynamic,the eontant is KA=2.1×10^7L·mol^-1,the binding sites number is 0.22.The synchronous fluorescence spectra of tyrosine residues did not demonstrate any change .But the intensity of tryptophane residues fluorescence was decreased.Suggesting that nicotine affect BSA fluorescence spectra though affecting tryptophane residues fluorescence.

关 键 词：尼古丁 牛血渍蛋白 荧光猝灭 

分 类 号：Q51[生物学—生物化学]