高效制备转基因小鼠的优化试验  

Optimization Experiment to Establish Transgenic Mice in High Efficiency

作　　者：谢夏阳[1] 滕勇[2] 刘丽均[1] 徐艺玫[3] 唐一岷[1] 徐平[1] 

机构地区：[1]中国科学院上海实验动物中心,上海201615 [2]中山大学生命科学学院,广州510275 [3]第一军医大学,广州510515

出　　处：《上海交通大学学报（农业科学版）》2006年第1期84-89,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：科技基础性工作专项资金资助2001DEA10001

摘　　要：为了优化转基因小鼠的制备技术,试验着重对注射DNA的制备、高质量受精卵的获得、显微注射、胚胎移植及早期胚胎检测等各步骤中的主要影响因素进行了优化。移植后出生的小鼠经PCR及Southern Blot检测,阳性鼠检出率由1.85%(2/108)增加到23.36%(25/107)。由此说明,通过注射DNA的改良、技术优化以及GFP在转基因动物中的应用,转基因效率可以得到明显提高,为高效制作转基因动物提供基础。To optimize the technique of transgenic mice making, in this research, the major steps such as the preparation of DNA fragments for microinjection ,the preparation of eggs with high quality, the microinjection and implantation and early embryos detection were optimized. The born mice were examined by PCR and Southern blot. The ratio of transgenic founder mice was improved from 1.85 % （2/108） to 23.36 % （25/107）. Therefore, the technical platform for transgenic research in our center has been established due to the great improvement of the efficiency of producing transgenic mice by optimized techniques and application of GFP in transgenic animals.

关 键 词：显微注射 胚胎移植 转基因小鼠 

分 类 号：S851.43[农业科学—预防兽医学] TS201.6[农业科学—兽医学]