IGFBP-3基因PCR-RFLP多态性与中国荷斯坦牛泌乳性状的相关分析  

PCR-RFLP Polymorphism of Insulin-like Growth Factor Binding Protein 3 Gene and Its Association with Milk Trait of Chinese Holstein

作　　者：张润锋[1] 陈宏[1] 雷初朝[1] 胡沈荣[1] 苏利红[1] 

机构地区：[1]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《中国畜牧杂志》2006年第3期9-11,共3页Chinese Journal of Animal Science

基　　金：江苏省高校自然科学基金项目(No.03KJD230216);陕西省自然科学基金(No.2004C122);西北农林科技大学拔尖人才支持计划项目;西北农林科技大学研究生教育创新计划项目(No.05YCH018)

摘　　要：以胰岛素样生长因子结合蛋白3(IGFBP-3)基因作为中国荷斯坦牛部分泌乳性状的候选基因,在对61头中国荷斯坦牛进行PC R-R FLP分析的基础上,对中国荷斯坦牛群体中IGFBP-3基因座多态性与泌乳性状进行相关分析。结果表明:IGFBP-3基因座对产奶量、乳蛋白率和体细胞评分的影响显著(P<0.05),IGFBP-3 BB型个体的305 d产乳量显著(P<0.05)高于AA型和AB型,BB型个体的乳蛋白率和体细胞评分显著(P<0.05)低于AB型。Insulin-like growth factor binding protein 3 traits in Chinese Holstein. The associations between （IGFBP-3） gene was regarded as candidate gene of partial milk PCR-RFLP genotypes of IGFBP-3 gene locus and milk relative traits in 61 Chinese Holstein were analyzed with PROC GLM of SAS. LS Means ANOVA showed that the effects of IGFBP-3 locus on milk yield, protein percentage and somatic cell scores reached a significant level （P 〈 0.05）. The 305 day milk yield of the individuals with IGFBP-3 BB genotype was significantly higher than those with IGFBP-3 AB or IGFBP-3 AA genotype. The protein percentage and somatic cell scores of the individuals with IGFBP-3 BB genotype was significantly lower than that of those with IGFBP-3 AB genotype.

关 键 词：畜牧学  IGFBP-3基因 PCR—RFLP 泌乳性状 中国荷斯坦牛 

分 类 号：S823.91[农业科学—畜牧学]