聚合酶链反应在我国各生态型鼠疫菌检测中的应用  

THE APPLICATION OF PCR METHOD TO DETECT Y. PESTIS OF DIFFERENT ECOTYPES IN CHINA

作　　者：张景林[1] 李宏伟[1] 王洪喜[1] 林景涛[1] 宝建喜[2] 岳明祥[2] 钟萍[2] 王武芳[2] 

机构地区：[1]全国鼠疫布氏菌病防治基地 [2]中国人民解放军89870部队防检所

出　　处：《中国地方病防治》1996年第1期5-6,共2页Chinese Journal of Control of Endemic Diseases

摘　　要：应用聚合酶链反应(PCR)对我国各生态型鼠疫菌进行检测,结果被试的37株鼠疫菌扩增产物经琼脂糖凝胶电泳分析均呈现单—DNA带,其分子量大小与预期结果相符合。而假结核菌、结肠炎菌等均未见扩增带。10cfu即可产生阳性反应,仅耗时4h。表明本法快速、特异、敏感,可用于各生态型鼠疫菌的快速诊断。Using PCR method examinated Y. pestis of different ecotypes in China. The amplified products of 37 strains of Y. pestis showed a single DNA band through agarose gel electrophoresis, and the size of molecule was corresponding to the desired result. When Y. pseudotuberculosis ,Y. enterocolitica were detected under the same condition no amplified fragments appeard. The sensitivity of the procedure is 10 cfu,and the whole course needed less than 4h. Therefore it is a speedy,special,sensitive method and suitable to the rapid diagnosis of Y. pestis of all kinds of e-cotypes.

关 键 词：鼠疫杆菌 医学检验 聚合酶链反应 

分 类 号：R378.61[医药卫生—病原生物学] R446.5[医药卫生—基础医学]