当归补血汤汤剂正丁醇部位中黄芪的HPLC指纹初步分析  

HPLC fingerprint of Radix astragali from n-Butanol extract of Danggui Buxue Decoction

作　　者：黄月纯[1] 魏刚[1] 黄水清[2] 刘东辉[2] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510015 [2]广州中医药大学,广东广州510405

出　　处：《中成药》2006年第2期165-168,共4页Chinese Traditional Patent Medicine

基　　金：广东省中医药管理局资助课题(103025)

摘　　要：目的:探讨当归补血汤汤剂(黄芪、当归)正丁醇部位中黄芪的HPLC指纹特征峰。方法:采用HPLC方法分析。采用Nuc leodur C18G ravity色谱柱;流动相为乙腈-水(梯度洗脱);检测波长为210 nm;流速为1 mL/m in。结果:复方煎剂正丁醇部位主要有14个特征峰,其中黄芪有14个特征峰,当归有3个特征峰。结论:黄芪药材、黄芪煎剂与复方煎剂中的主要特征峰的相对含量呈现一定的相关性。AIM ： To study the main components of the position of n-Butanol extract of Danggui Buxie Decoction （Radix Astragali, Radix Angelicae Sinensis）. METHODS： The main components were determined by HPLC. HPLC with Nucleodur C18 Gravity column was used, the Acetonitrile-water（ gradient elution） was used as a mobile phase and detecting wavelength at 210 nm. RESULTS： There were 14 common peaks in the peak position of n-Butanol extract of Danggui Buxue Decoction, Radix astragli had 14 peaks and Radix angelicae sinensis 3 peaks. CONCLUSION ： The main components of the peak position among n-Butanol extract of Radix astragali and its extract and Danggui Buxie Decoction have correlation.

关 键 词：当归补血汤 黄芪 指纹图谱 HPLC 

分 类 号：R927.1[医药卫生—药学]