中药桂枝的HPLC指纹图谱研究  

HPLC fingerprint of Ramulus cinnamomi

作　　者：林佳[1] 徐丽珍[1] 刘江云[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中成药》2006年第2期169-171,共3页Chinese Traditional Patent Medicine

基　　金：北京市自然科学基金重点项目(7011003)

摘　　要：目的:建立中药桂枝的指纹图谱,为其质量控制和药材鉴别提供理论依据。方法:高效液相色谱法,色谱柱:Krom a-sil C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.5%醋酸水溶液(梯度洗脱);检测波长:280 nm,温度:25°C,流速:1 mL/m in。结果:确定了桂枝HPLC中桂皮醛、肉桂酸、邻甲氧基肉桂酸、香豆素所对应的保留时间;不同产地的桂枝HPLC指纹图谱有很大的相似。结论:桂枝的HPLC指纹图谱可用于药材鉴别和质量控制。AIM： To establish HPLC fingerprint of Ramulus cinnamomi. This fingerprint was expected to be standards of quality control and identification of the Chinese crude drug. METHODS： The HPLC method was used, chromatography condition were Kromasil C 18 （250 mm ×4.6 mm, 5 μm） column with gradual mobile phase of acetonitrile-0.5% acetic acid, UV detection wavelength at 280 nm and column temperature at 25 ℃ with the flow rate of 1 ml · min^- 1. RESULTS ： The retention time of Cinnamaldehyder, Cinnamic acid, o-Methoxycinnamic acid and Coumarin in Ramulus cinnamomi were determined. The fingerprint showed high similitude in samples from different regions. CONCLUSION： The HPLC fingerprint of Ramulus cinnamomi, can be used as a standard of quality control and identification of the Chinese crude drug.

关 键 词：桂枝 指纹图谱 高效液相法 

分 类 号：R284.1[医药卫生—中药学]