电针对抑郁模型大鼠行为活动的影响  

Effect of Electroacupuncture on Behavioral Activities of Depression Rats

作　　者：俞瑾[1] 景政 刘琼[1] 汪军[1] 王彦青[1] 吴根诚[1] 

机构地区：[1]复旦大学上海医学院中西医结合系针刺原理研究所,上海200032 [2]山东省中医药学校针灸系,莱阳265200

出　　处：《针刺研究》2006年第1期31-34,共4页Acupuncture Research

基　　金：国家自然科学基金(30371798);教育部博士点科研基金(20020246043)资助

摘　　要：目的:观察电针及合用小剂量三环类抗抑郁药———氯丙咪嗪后抑郁模型大鼠行为活动的改善情况,探讨电针及针药结合的抗抑郁效果。方法:SD大鼠48只,分为正常组、模型组、电针组、小剂量氯丙咪嗪(2.5 mg/kg)组、大剂量氯丙咪嗪(5 mg/kg)组、电针合用小剂量氯丙咪嗪(2.5 mg/kg)组。采用国际通用的不可预知的长期应激刺激制造大鼠抑郁模型。应激刺激14 d后,治疗组(包括电针组和不同剂量的药物组)在给予应激刺激的同时开始治疗。药物治疗每天1次,共给药14次。电针取“百会”、右侧“阳陵泉”,疏密波(疏波频率4 Hz,串长2.5 s;密波频率60 Hz,串长5 s),强度≤1 mA,持续30 min/次,隔天1次,共治疗7次。采用活动性测试和强迫游泳实验评判大鼠抑郁程度的改善情况。结果:造模成功后,大鼠在活动性测试中垂直和水平运动显著下降,强迫游泳中静止时间显著增加。电针和大剂量的氯丙咪嗪(5 mg/kg)均可以增加大鼠的垂直和水平运动,减少强迫游泳实验中大鼠的静止时间。电针与小剂量氯丙咪嗪合用可进一步减少静止时间,增加垂直运动和水平运动。结论:电针具有良好的抗抑郁效果,小剂量药物合用电针一方面可降低用药剂量,另一方面可进一步提高电针疗效。Objective： To observe the effect of electreaoupuncture （EA） combined with Clomipramine （CIo） on behavioral activities of depression rats. Methods： Forty-eight SD rats were randomized into normal control, model, EA, Cio2.5mg, CIo 5 mg and EA＋ CIo 2.5 mg groups, with 8 cases in each group. Chronic unpredictable stress （CUS） model was established by forcing the rat to have fasting, cool-water swim, water-deprivation, high temperature environment, etc. in 28 clays. EA （4 Hz and 60 Hz, duration of the pulse train： 2.5 s and 5 s,≤1 mA） was applied to ＂Baihui＂ （GV 20） and right ＂Yanglingquan＂ （GB34） for 30 min, once every other clay, 7 times altogether. CHP was given to the animals by intraperitoneal injection. The open-field test was used to observe the rats＇ rearing and crossing activities, and the forced swim test was employed to determine the rats＇ duration of immovability （DI） during 5 min in a water pool. Results： In comparison with normal control group, the rearing and crossing mevement times of rats in model group decreased significantly （ P 〈 0.05） ; while compared with model group, the rearing times of rats in EA, CIo2.5 mg, CIo5 mg and EA＋CIo2.5 mg groups, and crossing movement times of rats in EA, CIo5 mg and EA＋ CIo 2.5 mg groups increased remarkably （P 〈 0.05）, and the effect of EA ＋ CIo 2.5 mg was significantly superior to those of EA and CIo2.5 mg groups in the rearing times and to that of EA group in the crossing times （P〈0.05）. In comparison with normal control group, the rats＇ DI in the water pool in model group prolonged significantly （P〈 0.05） ; while compared with model group, the DI of EA, CIo 5 mg and EA ＋ CIo 2.5 mg shortened significantly （P〈 0.05）, and the effect of EA ＋ CIo 2.5 mg group was significantly superior to that of EA group on the 28th clay （P 〈 0.05）. Conclusion： EA works well in relievingdepression, and EA combined with small dose of CIo can lower the dosage of CIo and raise the ef

关 键 词：抑郁症 慢性不可预知应激 电针 氯丙咪嗪 行为测试 

分 类 号：R245.97[医药卫生—针灸推拿学]