电针对“关元”穴区初级感觉传入神经元节段分布的影响  

Effects of Electroacupuncture on the Distribution of Primary Afferents from "Guanyuan"(CV 4) Area——A CB-HRP Tracing Study

作　　者：刘巧玲[1] 安晓飞[1] 吴根诚[1] 陈伯英[1] 

机构地区：[1]复旦大学上海医学院中西医结合系神经生物学教研室,上海200032

出　　处：《针刺研究》2006年第1期50-53,共4页Acupuncture Research

基　　金：中国博士后科学基金资助项目(No.2003033280)

摘　　要：目的：用CB—HRP示踪法对大鼠“关元”穴传入的神经节段分布进行研究。方法：SD大鼠随机分成对照组和电针组，分别在“关元”穴注入0．03％CB-HRP 20μL。注射后，电针组动物每天给予“关元”穴电针30min，共3d。84h后，观察两组动物的双侧相应节段的背根神经节（DRG）中阳性标记细胞的形态学变化和数量的变化。结果：在两组动物的T11～L3双侧DRG中，都存在有与“关元”穴相关的CB—HRP标记细胞；电针组的标记细胞总数显著高于对照组；标记细胞总数分布最多的节段，对照组在L3，电针组比对照组高一节段，在L2；两组标记细胞的形态学观察，均可分为大、中、小三型细胞，其中以小型细胞占多数。结论：针刺大鼠“关元”穴，可使穴位区局部感觉神经初级传入功能活动加强，L2可能是针刺“关元”穴信号传入脊髓的主要途径。Objective： The spinal segmental distribution of neural innervations related to ＂Guanyuan＂ （CV 4） in rats has been investigated by horseradish peroxidase conjugated cholera toxin B subunit （CB-HRP） tracing technique. Methods： Twelve SD female rats were divided randomly into electrcacupuncture group （EA） and control group （C）, CB-HRP solution （0.03 % 20μL） was injected into ＂Guanyuan＂ acupoint of the rats, The HRP labeled neurons were observed morphologically and counted in the related spinal dorsal root ganglia （DRG）. Results; The sensory nerve afferents of ＂Guanyuan＂ acupoint mainly projected to the neurons of T11 to L3 DRG. The labeled neurons were observed to be large, medium and small sized cells, of which the small size was in The majority. The number of labeled neurons in EA group was increased significantly compared with control group （P〈0.05）. The nerve fibers projected to L2 were activated by the EA treatment and the number of HRP labeled cells in L2 DRG was increased significantly, Conclusion： EA at ＂Guanyuan＂ acupoint may affect the local innervation activity, resulting in the projection of the local primary sensory afferents focusing on L2 DRG. So this study provides a new morphological evidence for transduction of acupuncture signals.

关 键 词：电针“关元”穴霍乱毒素B亚单位-辣根过氧化物酶(CB-HRP) 背根神经节(DRG) 

分 类 号：R338[医药卫生—人体生理学]