反相高效液相色谱法同时测定对苯氧基苯酚和对氯苯酚  

Determination of p-phenoxyphenol and p-chlorophenol by reverse-phase high performance liquid chromatography

作　　者：陶建伟[1] 高永红[1] 陆庆宁[1] 

机构地区：[1]上海应用技术学院化工系,上海200235

出　　处：《分析试验室》2006年第2期13-15,共3页Chinese Journal of Analysis Laboratory

基　　金：上海市农委重点攻关项目[沪农科攻字(2003)第7-8号]

摘　　要：建立了一种同时测定对苯氧基苯酚和对氯苯酚的反相高效液相色谱方法.采用Diamonsil（钻石）C18（250 mm×4.6 mm i.d.,5μm）色谱柱,以甲醇-水（V（甲醇）：V（水）=8：2）为流动相,在波长为230 nm处进行检测.对苯氧基苯酚和对氯苯酚在20～230μg/mL范围内,峰面积与质量浓度呈良好的线性关系,相对标准偏差RSD分别为0.26%、0.53%,回收率在99.0%～101%之间.方法可用于对苯氧基苯酚的合成工艺研究及成品质量控制.A method for the determination of p-phenoxyphenol and p-chlorophenol by reverse-phase high performance liquid chromatography was developed. Diamonsil C18 （250 mm × 4.6 mm i. d. ,5 μm） was chosen as analytical column, methanol-water （8：2） at a flow-rate of 0.5 mL/min as mobile phase with UV detection at 230 nm. The relationship between peak area and concentration in the range from 20 to 230 μg/mL shows a good linearity. The recovery was from 99.0% to 101% with the relative standard deviation of 0.26% and 0.53 %, respectively. This method is accu- rate, rapid, simple and reproducible, and suitable for the analysis in the sythesis process and the quality control of p- phenoxyphenol.

关 键 词：反相高效液相色谱法 对苯氧基苯酚 对氯苯酚 

分 类 号：O657.7[理学—分析化学]