毛细管电泳电化学检测法测定烟草中的多元酚  

Determination of polyphenols in tobacco by capillary electrophoresis with electrochemical detection

作　　者：彭友元[1] 叶建农[2] 李国清[1] 

机构地区：[1]泉州师范学院化学系,泉州362000 [2]华东师范大学化学系,上海200062

出　　处：《分析试验室》2006年第2期92-96,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20375013);福建省青年创新基金(2003J037);泉州师范学院专项课题(2001-2-22)

摘　　要：采用毛细管电泳电化学检测法同时测定了烟草中的多元酚,即芦丁、绿原酸,槲皮素和咖啡酸。考察了工作电极的氧化电位、运行缓冲溶液浓度和pH值,分离电压和进样时间对分离和检测的影响。在优化条件下,以300μm直径的碳圆盘电极为工作电极,检测电位为+0.9 V(vs.SCE),在50 mmol/L硼酸盐(pH 8.4)的运行缓冲液中,被测物浓度与峰电流在三个数量级范围内呈良好线性,检出限为2×10-7或5×10-7g/mL。方法有着良好的重现性,被测组分的迁移时间和峰高的相对标准偏差(RSDs)小于4%(n=7)。单次测定可在16 min内完成,已用于实际样品多元酚的测定,样品处理简单,无须预富集。a simultaneous determination of rutin, chlorogenic acid, quercetin and caffeic acid in tobacco by capillary electrophoresis with electrochemical detection （CE-ED） was reported. Effects of several important factors such as pH and running buffer concentration, separation voltage, injection time, and detection potential were investigated to acquire the optimum conditions. Under the optimum conditions, the analytes could be separated within 16 rain in a 75 cm length capillary at a separation voltage of 18 kV in a 50 mmol/L borate buffer （pH 8.4）. A 300 μm diameter carbon disk electrode generates good response at ＋ 0.90 V （ vs. SCE） for all analytes. The relationship between peak currents and analyte concentrations was linear over about three orders of magnitude with detection limits （S/N = 3） ranging from 2 × 10^-7 - 5 × 10^-7 g/mL for all analytes. This proposed method demonstrated long-term stability and reproducibility with relative standard deviations of less than 4 % for both migration time and peak current （ n = 7）. It has been successively used for the determination of these analytes in real samples, and the assay result was satisfactory.

关 键 词：毛细管电泳 电化学检测 烟草 多元酚 

分 类 号：O657.8[理学—分析化学]