薄层扫描法测定染料木素和大豆苷原的含量  

Determination of Genistein and Daidzein by TLCS

作　　者：刘燕华 陈光芝 褚新红 

机构地区：[1]德州泰康药业有限公司,德州253000 [2]山东三株实业有限公司,济南250100

出　　处：《齐鲁药事》2006年第2期98-99,共2页qilu pharmaceutical affairs

摘　　要：目的建立染料木素和大豆苷原的薄层扫描测定方法。方法薄层色谱条件是:吸附剂:聚酰胺-6薄膜,展开剂:苯-丁酮-甲醇(3∶1∶0.5),结果染料木素和大豆苷原均在250nm处有最大吸收,本法线性关系良好(染料木素:r=0.9993;大豆苷原:r=0.9995)。染料木素的平均回收率98.52%(n=5);大豆苷原的平均回收率99.45%(n=5)。结论方法简便,准确,重现性好,可用于测定染料木素和大豆苷原的含量。OBJECTIVE To establish a TLC scanning （TLCS）method for determination of genistein and daidzein. METHODS The conditions of the TLC were as follow：The absorber was Polyamide-6 diaphragm. The mobile phase was benzene-butanone-ethanol（3 ： 1 ： 0.5）. RESULTS Genistein and daidzein had the max absorption in 250nm and the method has a good linear dependence（Genistein： r = 0. 9993 ,daidzein： r = 0. 9995）. The average recovery was 98.52%, RSD = 1. 90% （genistein; n =5） and 99.45%, RSD =2.49% （ daidzein, n =5） . CONCLUSION The method was convenient,correct,reliable and suitable for determination of genistein and daidzein.

关 键 词：薄层扫描 染料木素 大豆苷原 

分 类 号：R927.2[医药卫生—药学]