外周血T细胞受体和CD_(25)在大鼠心脏移植排斥反应中的变化  

The expression of T cell receptor and CD_(25) on peripheral lymphocytes in cardiac allograft rejection of rat

作　　者：周永列[1] 严志焜[1] 吕治林[1] 赵仲生[1] 邱莲女[1] 周冰[1] 潘晓华[1] 林惠君[1] 

机构地区：[1]浙江省人民医院,浙江省器官移植重点研究实验室心脏移植分室,杭州310014

出　　处：《浙江医学》2006年第2期106-109,共4页Zhejiang Medical Journal

基　　金：浙江省科技厅科研基金资助(基金编号:011103025)

摘　　要：目的探讨T细胞受体(TCR)和IL-2受体α链(CD25)在大鼠心脏移植排斥中的作用及意义。方法用流式细胞术检测大鼠异位心脏移植术前和术后不同时间外周血淋巴细胞TCRαβ、TCRγδ和CD25的表达变化,结果心脏移植术后淋巴细胞TCRαβ和CD25的表达[(52.0±10.7)%、(2.1±1.9)%]显著高于术前[(41.7±9.2)%、(1.3±0.8)%](均P<0.05);TCRαβ和CD25的表达变化与排斥反应的进程有关,术后第5天[(51.8±11.5)%、(2.0±1.5)%]显著高于术前(均P<0.05),至第14天[(60.5±6.3)%、(3.1±1.6)%]仍维持在高峰平台,高于术前(均P<0.05),此时移植物排斥反应多表现为2～3级。TCRδγ在外周血中的表达量较低,在术前和术后无显著变化。结论淋巴细胞TCRαβ和CD25在移植排斥反应中可能发挥重要的作用,动态监测外周血淋巴细胞TCRαβ和CD25的表达有助于临床移植排斥反应的评价。Objective To explore the significance of T cell receptor （TCR）and interleukin-2 receptor （CD25）in allograft rejection. Methods The expression of TCRαβ, TCRγδ and CD25 in peripheral lymphocytes were detected by flow cytometry preoperatively and 3,5,7,14 days postoperatively in rat cardiac allograft recipients. Results The levels of TCRαβ and CD25 in peripheral lymphocytes before operation were significantly higher than that after operation. The changes of TCRαβ and CD25 were associated with the course of rejection. The peak value of TCRαβ and CD25 was observed at the 14th day after operation, meanwhile the degree of graft rejection was moderate （grade 2 to grade 3）. The TCRγδ expression on lymphocytes was not different before and after operation. Conclusion The results suggests that TCRαβ and CD25 may play important roles in the course of rejection and might be a useful indicator for diagnosis of acute cardiac allograft rejection.

关 键 词：T细胞受体 CD25 心脏移植 移植物排斥 大鼠 

分 类 号：R654.2[医药卫生—外科学] R593.241[医药卫生—临床医学]