HPLC法测定灯盏花素脂质体中灯盏乙素的含量  

HPLC Method for Assay of Breviscapine Liposome

作　　者：高展[1] 黄罗生[1] 赵浩如[1] 

机构地区：[1]中国药科大学中药研究所中药制剂研究室,南京210038

出　　处：《海峡药学》2006年第1期58-61,共4页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC测定灯盏花素脂质体中药物舍量的定量方法。方法色谱柱：DIKMA Diamonsil（TM）C185μm4．6×250mm．流动相；甲醇-四氩咦喃-0．1％磷酸（14：14：72）．检测波长。335nm；拄温：40℃。结果在此色谱条件下灯盏乙素与辅料及溶剂峰均得到良好的分离．灯盏乙素在5-100μg·mL^-1。范围内线性良好（r=0．9999），平均加样回收率为102．1％．RSD为1．26％。结论谊方法简便准确，专属性强．拈满足谊制荆质量标准的要求。OBJECTIVE To establish an RP-HPLC method to determine the drug substance content of Breviscapine Liposomes. METHODS Using methanol-tetrahydrofuran-0. 1% phosphoric acid （14 ： 14 ： 72） as mobile phase,the separation was performed on a DIKMA Diamonsil （TM） C18（5μm 4.6 × 250nm） column with a flow rate of 1.0mL · min^-1. The detective wavelength was 335nm and the column temperature was 40℃. RESULTS Peaks for scutellarin,excipients or solvents were separated well in the chromatographic file with the RP-HPLC method. Regression analysis of the data of scutellarin concentration against its peak area showed good straight line over the range. 5 - 100 μg · mL^-1 （r= 0. 9999）. And the average recovery was 102.1%. CONCLUSION The method was simple,specific and accurate,and can effectively control quality of Dangguisini granules.

关 键 词：灯盏花素膳质体 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]