干滤纸血片用于日本血吸虫病患者抗体检测的现场应用研究  

Field Application of Whole Blood Absorbed on Filter Paper to Detcet Antibody of Individual Infected with Schistosomiasis Japonicum

作　　者：郑莉[1] 杨光斌[1] 李秋萍[1] 唐丽[1] 魏凤华[1] 徐兴建[1] 

机构地区：[1]湖北省疾病预防控制中心,武汉430079

出　　处：《公共卫生与预防医学》2006年第1期17-19,共3页Journal of Public Health and Preventive Medicine

摘　　要：目的评价干滤纸血片用于现场检测日本血吸虫病患者抗体的应用价值。方法现场选取湖北省2个血吸虫病流行区。对6～60岁居民同时采集静脉血分离血清和微量末梢血滤纸血片．均进行IHA与ELISA检测;对两种采样方法成本费用进行比较和居民依从性进行调查。结果在现场同等条件下，两种样本IHA与ELISA阳性检出率均无显著性差异（P〉0．05）;采集滤纸血片样本比静脉血清成本低1．2元／人份；受检者对滤纸血片采样法的依从性（52．1％）显著高于静脉抽血（21．3％）。蛄论干滤纸血片法操作简便、快速、费用低，受检者依从性高，适合于现场大规模血吸虫病普查和流行病学调查。Objective To assess the practical value of the whole blood absorbed on filter paper to detect antibody of Schistosomiasis japonica for the examination in the filed. Methods Serum samples were obtained by venepuncture and finger-prick blood samples from the same individual （6-60 years old） and were directly absorbed on filter paper from two Schistosomiasis endemic areas in Hubei Province. IHA and ELISA test using serum and filter paper blood were compared. The comparison of the cost and the compliance of the two sample-collecting ways was investigated. Results There was no significant difference between the two methods in the same situation. The cost of collecting whole blood absorbed on filter paper for one person was about ￥1.2 yuan per person. The compliance of the individual for whole blood absorbed on filter paper（52. 1%） was higher than that of serum（21.3%）. Conclusion Collecting whole blood absorbed on filter paper was easier, faster, less expensive and of more compliance than that of serum, it was suitable for application in field survey.

关 键 词：血吸虫病 滤纸血片 IHA ELISA 现场 

分 类 号：R532.21[医药卫生—内科学]