人表皮角质形成细胞中PAI-3/PCI的表达及作用  

Expression and role of plasminogen activator inhibitor/protein C inhibitor in keratinocytes of human epidermis

作　　者：张诚[1] 李习玲[1] 连小华[1] 王韵[1] 杨珂[1] 余谨[1] 高强国[1] 杨恬[1] 

机构地区：[1]第三军医大学基础医学部细胞生物学教研室,重庆400038

出　　处：《第三军医大学学报》2006年第4期294-297,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30270671;30371383)~~

摘　　要：目的探讨人表皮角质形成细胞(KERATINOCYTE,KC)中,PAI-3/PCI的表达及作用。方法利用免疫细胞化学、RT-PCR方法,检测高CA2+浓度作用下,培养的人分化表皮KC及KC提取物角质壳中PAI-3的表达变化。结果高CA2+浓度下培养24H后,分化标记物K10弱阳性表达,PAI-3呈阳性表达;培养48H后,K10强阳性表达,而PAI-3的阳性表达明显增加,72H后,表达开始降低,在低CA2+浓度下阳性染色主要位于细胞核膜周围而高CA2+浓度下主要位于细胞质,同时,PAI-3存在终末分化KCS角质壳中。结论人表皮KC分化过程中,PAI-3的表达随着分化的增加而增加,PAI-3存在终末分化KCS角质壳蛋白中,表明PAI-3参与KC分化的作用。Objective To explore the expression and role of PAI-3/PCI in keratinocytes （KCs） of human epidermis. Methods The immunocytochemical method （ICC） and RT-PCR were used to determine the PAI-3 amounts in the differentiated human KCs and in the abstraction of cornified envelope of KCs cultured in high calcium medium. Results In KCs cultured for 24 h in high calcium medium, the KC differentiation marker keratin 10 （K10） was slightly expressed, and a large amount of PAI-3 was detected. An amount increase of PAI-3 and K10 was observed after the KCs were cultured for 48 h in high calcium medium, but at 72 h the exprssion of PAI-3 decreased. The positive stain of PAI-3 was mainly localized in perinuclear membrane in low calcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope of terminally differentiated keratinocytes. Conclusion PAI-3 expression increased in the processes of KCs differentiation. PAI-3 can incorporate into the cornified envelope and may promote the differentiation of keratinocytes.

关 键 词：角质形成细胞 分化 PAI-3 角质壳 PCI 

分 类 号：R322.991[医药卫生—人体解剖和组织胚胎学] R329.26[医药卫生—基础医学]